High fat diet reduces the expression of miRNA‐29b in heart and increases susceptibility of myocardium to ischemia/reperfusion injury. Issue 6 (26th October 2018)

• Record Type: Journal Article
• Authors: Guedes, Elaine Castilho; da Silva, Ivson Bezerra; Lima, Vanessa Morais; Miranda, Juliane B.; Albuquerque, Rudá P.; Ferreira, Julio C. B.; Barreto‐Chaves, Maria Luiza M.; Diniz, Gabriela Placoná
• Is Part Of: Journal of cellular physiology. Volume 234:Issue 6(2019:Jun.)
• Page Start: 9399
• Page End: 9407
• Publication Date: 2018-10-26
• Subjects: heart -- hypoxia -- ischemia/reperfusion injury -- miRNA‐29b -- obesity; Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.27624
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract: Several studies have shown the role of microRNAs (miRNAs) in myocardial dysfunction in response to ischemia/reperfusion (I/R). In this study, we investigated the impact of high fat (HF) diet in the myocardial susceptibility to I/R injury, as well as in the expression of miRNA‐29b. Isolated heart experiments using the ex vivo Langendorff perfusion model were used to induce cardiac I/R injury. HF diet‐induced cardiac hypertrophy and impaired cardiac functional recovery after I/R. miRNA‐29b, which targets Col1, was reduced in the heart of HF diet‐fed mice, whereas the cardiac expression of Col1 was increased. In addition, hypoxia–reoxygenation (H/R) reduced the expression of miRNA‐29b in cardiomyoblasts cultures. However, the overexpression of miRNA‐29b in cardiomyoblasts reduced p53 mRNA levels and H/R injury, suggesting that downregulation of miRNA‐29b may be involved in I/R injury. Together, our findings suggest that the reduced expression of miRNA‐29b may be involved in the deteriorated cardiac functional recovery following I/R in obese mice. Abstract : Obese mice displayed deteriorated cardiac functional recovery following the ischemia/reperfusion (I/R) injury, which was accompanied by the reduced expression of microRNA (miRNA)‐29b. Hypoxia–reoxygenation (H/R) reduced the expression of miRNA‐29b in cardiomyoblasts cultures. The overexpression of miRNA‐29b attenuated the increased cell death in response to H/R, suggesting that the reduced expression of miRNA‐29b may be involved in the deteriorated cardiac functional recovery following the I/R in obese mice.