Sauropus brevipes ethanol extract negatively regulates inflammatory responses in vivo and in vitro by targeting Src, Syk and IRAK1. (1st January 2021)

• Record Type: Journal Article
• Authors: Kim, Ji Hye; Park, Jae Gwang; Hong, Yo Han; Shin, Kon Kuk; Kim, Jin Kyeong; Kim, Young-Dong; Yoon, Ki Dong; Kim, Kyung-Hee; Yoo, Byong Chul; Sung, Gi-Ho; Cho, Jae Youl
• Is Part Of: Pharmaceutical biology. Volume 59:Number 1(2021)
• Page Start: 74
• Page End: 86
• Publication Date: 2021-01-01
• Subjects: Natural product medicine -- anti-inflammatory remedy -- flavonoids -- gastritis -- peritonitis; Pharmacognosy -- Periodicals; Materia medica, Vegetable -- Periodicals; 615.321
• DOI: 10.1080/13880209.2020.1866024
• Languages: English
• ISSNs: 1388-0209

Abstract

Abstract: Context: Sauropus brevipes Müll. Arg. (Phyllanthaceae) has been used as an effective ingredient in a decoction for the treatment of diarrhoea. However, there was no report on its modulatory role in inflammation. Objective: This study investigates anti-inflammatory effect of S. brevipes in various inflammation models. Materials and methods: The aerial part of S. brevipes was extracted with 95% ethanol to produce Sb-EE. RAW264.7 cells pre-treated with Sb-EE were stimulated by lipopolysaccharide (LPS), and Griess assay and PCR were performed. High-performance liquid chromatography (HPLC) analysis, luciferase assay, Western blotting and kinase assay were employed. C57BL/6 mice (10 mice/group) were orally administered with Sb-EE (200 mg/kg) once a day for five days, and peritonitis was induced by an intraperitoneal injection of LPS (10 mg/kg). ICR mice (four mice/group) were orally administered with Sb-EE (20 or 200 mg/kg) or ranitidine (positive control) twice a day for two days, and EtOH/HCl was orally injected to induce gastritis. Results: Sb-EE suppressed nitric oxide (NO) release (IC50 =34 µg/mL) without cytotoxicity and contained flavonoids (quercetin, luteolin and kaempferol). Sb-EE (200 µg/mL) reduced the mRNA expression of inducible NO synthase (iNOS). Sb-EE blocked the activities of Syk and Src, while inhibiting interleukin-1 receptor associated kinases (IRAK1) by 68%. Similarly, orally administered Sb-EE (200 mg/kg) suppressed NO production by 78% and phosphorylation of Src and Syk in peritonitis mice. Sb-EE also decreased inflammatory lesions in gastritis mice. Discussion and conclusions: This study demonstrates the inhibitory effect of Sb-EE on the inflammatory response, suggesting that Sb-EE can be developed as a potential anti-inflammatory agent.