Kazak faecal microbiota transplantation induces short-chain fatty acids that promote glucagon-like peptide-1 secretion by regulating gut microbiota in db/db mice. (1st January 2021)

• Record Type: Journal Article
• Authors: Han, Xue; Wang, Ye; Zhang, Peipei; Zhu, Manli; Li, Ling; Mao, Xinmin; Sha, Xiaoting; Li, Linlin
• Is Part Of: Pharmaceutical biology. Volume 59:Number 1(2021)
• Page Start: 1075
• Page End: 1085
• Publication Date: 2021-01-01
• Subjects: Kazak individuals -- type 2 diabetes mellitus -- short-chain fatty acids-producing bacteria; Pharmacognosy -- Periodicals; Materia medica, Vegetable -- Periodicals; 615.321
• DOI: 10.1080/13880209.2021.1954667
• Languages: English
• ISSNs: 1388-0209

Abstract

Abstract: Context: Faecal microbiota transplantation (FMT) from Kazak individuals with normal glucose tolerance (KNGT) significantly reduces plasma glycolipid levels in type 2 diabetes mellitus db/db mice. However, the mechanism behind this effect has not been reported. Objective: To study the mechanism of improved glycolipid disorders in db/db mice by FMT from a KNGT donor. Materials and methods: The normal diet group consisted of db/m mice orally administered 0.2 mL phosphate buffer saline (PBS) ( db/m + PBS ). For the db/db + PBS (Vehicle) and db/db + KNGT (FMT intervention group) groups, db/db mice received oral 0.2 mL PBS or faecal microorganisms from a KNGT donor, respectively. All mice were treated daily for 0, 6 or 10 weeks. Faecal DNA samples were sequenced and quantified using 16S rRNA gene sequencing and RT-qPCR, respectively. Short-chain fatty acid (SCFA) levels in the mouse faeces were determined by gas chromatography. G protein-coupled receptor 43 (GPR43) and glucagon-like peptide-1 (GLP-1) expression levels were determined. Results: FMT intervention significantly increased the relative abundance of Bacteroides uniformis (0.038%, p < 0.05). Clostridium levels (LogSQ) were increased ( p < 0.01), while Mucispirillum schaedleri levels (LogSQ) were decreased ( p < 0.01). Acetate and butyrate levels in the faeces were significantly increased (acetate; butyrate: 22.68 ± 1.82 mmol/L; 4.13 ± 1.09 mmol/L, p < 0.05). GPR43 mRNA expression and GLP-1 protein expression increased in colon tissue ( p < 0.05). Discussion and conclusions: Mechanistically, FMT-KNGT could improve glycolipid disorders by changing the bacterial composition responsible for producing SCFAs and activating the GPR43/GLP-1 pathway.