Incorporated‐bFGF polycaprolactone/polyvinylidene fluoride nanocomposite scaffold promotes human induced pluripotent stem cells osteogenic differentiation. Issue 10 (13th May 2019)

• Record Type: Journal Article
• Authors: Abazari, Mohammad Foad; Soleimanifar, Fatemeh; Enderami, Seyed Ehsan; Nematzadeh, Mahsa; Nasiri, Navid; Nejati, Fatemeh; Saburi, Ehsan; Khodashenas, Shabanali; Darbasizadeh, Behzad; Khani, Mohammad Mehdi; Ghoraeian, Pegah
• Is Part Of: Journal of cellular biochemistry. Volume 120:Issue 10(2019)
• Page Start: 16750
• Page End: 16759
• Publication Date: 2019-05-13
• Subjects: basic fibroblast growth factor -- nanofibers -- osteogenesis -- polycaprolactone -- polyvinylidene fluoride; Cytochemistry -- Periodicals; 572
• DOI: 10.1002/jcb.28933
• Languages: English
• ISSNs: 0730-2312

Abstract

Abstract : Bioactive scaffolds that can increase transplanted cell survival time at the defect site have a great promising potential to use clinically since tissue regeneration or secretions crucially depend on the transplanted cell survival. In this study embedded basic fibroblast growth factor (bFGF)‐polycaprolactone‐polyvinylidene fluoride (PCL‐PVDF) hybrid was designed and fabricated by electrospinning as a bio‐functional nanofibrous scaffold for bone tissue engineering. After morphological characterization of the PCL‐PVDF (bFGF) scaffold, nanofibers biocompatibility was investigated by culturing of the human induced pluripotent stem cells (iPSCs). Then, the bone differentiation capacity of the iPSCs was evaluated when grown on the PCL‐PVDF and PCL‐PVDF (bFGF) scaffolds in comparison with culture plate as a control using evaluating of the common osteogenic markers. The viability assay displayed a significant increase in iPSCs survival rate when grown on the bFGF content scaffold. The highest alkaline phosphatase activity and mineralization were detected in the iPSCs while grown on the PCL‐PVDF (bFGF) scaffolds. Obtained results from gene and protein expression were also demonstrated the higher osteoinductive property of the bFGF content scaffold compared with the scaffold without it. According to the results, the release of bFGF from PCL‐PVDF nanofibers increased survival and proliferation rate of the iPSCs, which followed by an increase in its osteogenic differentiation potential. Taking together, PCL‐PVDF (bFGF) nanofibrous scaffold demonstrated that can be noted as a promising candidate for treating the bone lesions by tissue engineering products. Abstract : Embedded basic fibroblast growth factor (bFGF)‐polycaprolactone‐polyvinylidene fluoride PCL‐PVDF hybrid was designed and fabricated by electrospinning, after that, morphology and biocompatibility of the scaffolds characterized by SEM and MTT assays and then osteoinductivity of PCL‐PVDF and PCL‐PVDF (bFGF) scaffolds were investigated while human induced pluripotent stem cells were grown on them—MTT and SEM results confirmed morphology and biocompatibility of the nanofibers—Highest osteoinductivity detected in PCL‐PVDF (bFGF) by gene and protein evaluation