Relationship Between O-GlcNAcase Expression and Prognosis of Patients With Osteosarcoma. Issue 1 (January 2022)

• Record Type: Journal Article
• Authors: Sombutthaweesri, Thamonwan; Wu, Shuangjiang; Chamusri, Nutchapon; Settakorn, Jongkolnee; Pruksakorn, Dumnoensun; Chaiyawat, Parunya; Sastraruji, Thanapat; Krisanaprakornkit, Suttichai; Supanchart, Chayarop
• Is Part Of: Applied immunohistochemistry & molecular morphology. Volume 30:Issue 1(2022)
• Publication Date: 2022-01
• Subjects: immunohistochemistry -- O-GlcNAcase -- O-GlcNAcylation -- O-linked N-acetylglucosamine transferase -- osteosarcoma; Diagnostic immunohistochemistry -- Periodicals; Immunohistochemistry -- Periodicals; Cells -- Morphology -- Periodicals; Molecular diagnosis -- Periodicals; 616.079
• DOI: 10.1097/PAI.0000000000000970
• Languages: English
• ISSNs: 1541-2016

Abstract

Abstract : Several studies have demonstrated a role of O -GlcNAcylation ( O -GlcNAc) in tumorigenesis of various carcinomas by modification of tumor-associated proteins. However, its implication in the pathogenesis of osteosarcoma remains unclear. This study aimed to investigate the levels of O -GlcNAc and the expressions of O- linked N- acetylglucosamine transferase (OGT) and O -GlcNAcase (OGA) in human osteosarcoma tissues, by using immunohistochemistry; and to find correlations between the levels or expressions and several clinicopathologic parameters. There were 109 first diagnosed osteosarcoma patients, including Enneking stage IIB (n=70) and III (n=39). Correlations between the immunoreactive score (IRS) and clinicopathologic parameters, overall survival, and metastasis-free survival were evaluated. A positive correlation was found between the IRS of OGA and the percentage of postchemotherapeutic tumor necrosis ( r =0.308; P =0.017). Univariate analysis revealed significantly lower OGA IRS in metastatic patients ( P =0.020) and poor chemotherapeutic-responder patients ( P =0.001). By multivariate analysis, presence of tumor metastasis ( P =0.002) and lower OGA IRS ( P =0.004) was significantly associated with shorter overall survival. Subgroup analysis in stage IIB osteosarcoma (n=70) demonstrated that male sex ( P =0.019), presence of tumor recurrence ( P =0.026), poor chemotherapeutic responder ( P =0.022), and lower OGA IRS ( P =0.019) were significantly correlated with short metastasis-free survival. But, lower OGA IRS was the only independent predictor for short metastasis-free survival ( P =0.006). Our findings suggested that O -GlcNAc pathway, especially OGA, may involve in pathogenesis and aggressiveness of osteosarcoma. Low level of OGA expression may be used as a poor prognostic indicator. Abstract : Supplemental Digital Content is available in the text.