MiR‐22enhances the radiosensitivity of small‐cell lung cancer by targeting theWRNIP1. Issue 10 (12th June 2019)

• Record Type: Journal Article
• Authors: Jiang, Wenhua; Han, Xuemei; Wang, Jingrui; Wang, Lin; Xu, Zanmei; Wei, Qiao; Zhang, Wenpan; Wang, Haitao
• Is Part Of: Journal of cellular biochemistry. Volume 120:Issue 10(2019)
• Page Start: 17650
• Page End: 17661
• Publication Date: 2019-06-12
• Subjects: DEGs -- miR‐22 -- radiosensitivity -- SCLC -- WRNIP1; Cytochemistry -- Periodicals; 572
• DOI: 10.1002/jcb.29032
• Languages: English
• ISSNs: 0730-2312

Abstract

Abstract: Small‐cell lung cancer (SCLC) is an aggressive malignancy characterized by high cellular proliferation and early distant metastasis. Our study aimed to explore the effect of miR‐22‐3p ( miR‐22, for short) on SCLC radiosensitivity and its molecular mechanisms. The expression level of miR‐22 was evaluated in a human normal lung epithelial cell line and a human SCLC cell line, and cell apoptosis and migration were detected. The expression of the miR‐22 direct target WRNIP1 mRNA and protein were explored. Five differentially expressed genes were detected. The miR‐22 expression in NCI‐H446 was significantly decreased, and miR‐22 overexpression significantly promoted cell apoptosis. miR‐22 overexpression could significantly inhibit the cell migration of SCLC cells, and miR‐22 had a negative regulatory effect on WRNIP1 mRNA and protein levels. KLK8 was downregulated, and the messenger RNA (mRNA) of four other genes ( PC, SCUBE1, STC1, and GPM6A ) was upregulated mRNA in cells overexpressing miR‐22, which was in accordance with the bioinformatics analysis. miR‐22 could enhance the radiosensitivity of SCLC by targeting WRNIP1 . Abstract : 1. miR‐22 overexpression strongly promoted small‐cell lung cancer (SCLC) cell apoptosis upon γ‐ray treatment. 2. miR‐22 overexpression can significantly inhibit the cell migration of SCLC cells under γ‐ray treatment. 3. miR‐22 is a direct negative regulatory of WRNIP1. 4. The differentially expressed gene (DEG) KLK8 downregulated messenger RNA (mRNA) and other four DEGs upregulated mRNAs in miR‐22 overexpression.