A microfluidic cytometer for white blood cell analysis. Issue 11 (9th August 2021)

• Record Type: Journal Article
• Authors: Peng, Tao; Su, Xinyue; Cheng, Xingzhi; Wei, Zewen; Su, Xuantao; Li, Qin
• Other Names: Su Xuantao guestEditor.
• Is Part Of: Cytometry. Volume 99:Issue 11(2021)
• Page Start: 1107
• Page End: 1113
• Publication Date: 2021-08-09
• Subjects: 3D hydrodynamic focusing -- airborne microlens -- microfluidic cytometer -- white blood cells classification; Flow cytometry -- Periodicals; Imaging systems in biology -- Periodicals; Imaging systems in medicine -- Periodicals; Diagnostic imaging -- Periodicals; 571.605
• DOI: 10.1002/cyto.a.24487
• Languages: English
• ISSNs: 1552-4922

Abstract

Abstract: Despite the wide use of cytometry for white blood cell classification, the performance of traditional cytometers in point‐of‐care testing remains to be improved. Microfluidic techniques have been shown with considerable potentials in the development of portable devices. Here we present a prototype of microfluidic cytometer which integrates a three‐dimensional hydrodynamic focusing system and an on‐chip optical system to count and classify white blood cells. By adjusting the flow speed of sheath flow and sample flow, the blood cells can be horizontally and vertically focused in the center of microchannel. Optical fibers and on‐chip microlens are embedded for the excitation and detection of single‐cell. The microfluidic chip was validated by classifying white blood cells from clinical blood samples.