Characterization of a hydrogen-producing bacterium Clostridium sp. 5A-1. Issue 6 (3rd May 2021)

• Record Type: Journal Article
• Authors: Cai, Jinling; Wang, Renyao; Wu, Qi; Wang, Guangce; Deng, Chaobing
• Is Part Of: International journal of green energy. Volume 18:Issue 6(2021)
• Page Start: 624
• Page End: 633
• Publication Date: 2021-05-03
• Subjects: Biohydrogen production -- dark-fermentation -- Clostridium -- pure culture -- sugar plant; Power resources -- Research -- Periodicals; Energy industries -- Periodicals; Energy development -- Periodicals; 333.79
• DOI: 10.1080/15435075.2021.1875469
• Languages: English
• ISSNs: 1543-5075

Abstract

ABSTRACT: Fermentative hydrogen production is considered as a promising source of renewable energy, and bacterial strain is an essential factor influencing hydrogen production. In this study, a high hydrogen-producing fermentative strain was isolated from a sugar plant, and identified as Clostridium sp. 5A-1 by 16S rRNA analysis. Strain 5A-1 was found to be a robust hydrogen-producing fermentative bacterium, which could produce hydrogen under a wide range of temperature (25°C–50°C) and initial pH (pH 5–9). The optimum culture temperature and initial pH for hydrogen production was 43°C and pH 7.5, respectively. The glucose concentration of 10 g/L resulted in the highest hydrogen production (1847 mL/L) and hydrogen production rate (231 mL/L.h), and 7 g/L glucose was the optimum substrate concentration for hydrogen yield (1.92 mol/mol glucose). Thus, Clostridium sp. 5A-1 is a potential candidate for fermentative hydrogen production.