FELASA guidelines for the refinement of methods for genotyping genetically-modified rodents: A report of the Federation of European Laboratory Animal Science Associations Working Group. (July 2013)

• Record Type: Journal Article
• Authors: Bonaparte (Convenor), Dolores; Cinelli, Paolo; Douni, Eleni; Hérault, Yann; Maas, Alex; Pakarinen, Pirjo; Poutanen, Matti; Lafuente, Mirentxu Santos; Scavizzi, Ferdinando
• Is Part Of: Laboratory animals. Volume 47:Number 3(2013)
• Page Start: 134
• Page End: 145
• Publication Date: 2013-07
• Subjects: genotyping -- husbandry -- polymerase chain reaction -- refinement -- sampling; Animal experimentation -- Periodicals; Animal models in research -- Periodicals; Laboratory animals -- Periodicals; Animal welfare -- Periodicals; 636.0885
• DOI: 10.1177/0023677212473918
• Languages: English
• ISSNs: 0023-6772

Abstract

The use of genetically-modified (GM) animals as research models continues to grow. The completion of the mouse genome sequence, together with the high-throughput international effort to introduce mutations across the mouse genome in the embryonic stem (ES) cells (www.knockoutmouse.org ) facilitates an efficient way to obtain mutated mouse strains as research models. The increasing number of available mutated mouse strains and their combinations, together with the increasing complexity in the targeting approaches used, reinforces the need for guidelines that will provide information about the mouse strains and the robust and reliable methods used for their genotyping. This information, however, should be obtained with a method causing minimal discomfort to the experimental animals. We have, therefore, compiled the present document which summarizes the currently available methods for obtaining genotype information. It provides updated guidelines concerning animal identification, DNA sampling and genotyping, and the information to be kept and distributed for any mutated rodent strain.