Resolving diurnal dynamics of the chloroplastic glutathione redox state in Arabidopsis reveals its photosynthetically derived oxidation. Issue 5 (24th February 2021)

• Record Type: Journal Article
• Authors: Haber, Zechariah; Lampl, Nardy; Meyer, Andreas J; Zelinger, Einat; Hipsch, Matanel; Rosenwasser, Shilo
• Is Part Of: The Plant Cell. Volume 33:Issue 5(2021)
• Page Start: 1828
• Page End: 1844
• Publication Date: 2021-02-24
• DOI: 10.1093/plcell/koab068
• Languages: English
• ISSNs: 1040-4651

Abstract

Abstract: Plants are subjected to fluctuations in light intensity, and this might cause unbalanced photosynthetic electron fluxes and overproduction of reactive oxygen species (ROS). Electrons needed for ROS detoxification are drawn, at least partially, from the cellular glutathione (GSH) pool via the ascorbate–glutathione cycle. Here, we explore the dynamics of the chloroplastic glutathione redox potential (chl- E GSH ) using high-temporal-resolution monitoring of Arabidopsis ( Arabidopsis thaliana ) lines expressing the reduction–oxidation sensitive green fluorescent protein 2 (roGFP2) in chloroplasts. This was carried out over several days under dynamic environmental conditions and in correlation with PSII operating efficiency. Peaks in chl- E GSH oxidation during dark-to-light and light-to-dark transitions were observed. Increasing light intensities triggered a binary oxidation response, with a threshold around the light saturating point, suggesting two regulated oxidative states of the chl- E GSH . These patterns were not affected in npq1 plants, which are impaired in non-photochemical quenching. Oscillations between the two oxidation states were observed under fluctuating light in WT and npq1 plants, but not in pgr5 plants, suggesting a role for PSI photoinhibition in regulating the chl- E GSH dynamics. Remarkably, pgr5 plants showed an increase in chl- E GSH oxidation during the nights following light stresses, linking daytime photoinhibition and nighttime GSH metabolism. This work provides a systematic view of the dynamics of the in vivo chloroplastic glutathione redox state during varying light conditions. Abstract : Monitoring the daily in vivo dynamics of the chloroplastic GSH redox state in light-stressed wild-type plants versus photoprotective mutants provides insight into the photosynthesis-dependent production of oxidants.