Draft genome of Sporosarcina sp. NCCP-2222T isolated from rhizosphere of mangrove plant Ceriops tagal reveals its potential as a plant biostimulant and constitutes a novel species. (January 2023)

• Record Type: Journal Article
• Authors: Bushra, Rabia; Uzair, Bushra; Manzoor, Sadia; Ahmed, Iftikhar
• Is Part Of: Biocatalysis and agricultural biotechnology. Number 47(2023)
• Publication Date: 2023-01
• Subjects: Ceriops tagal -- Mangroves rhizosphere -- Plant growth promoting bacteria -- Sporosarcina; digi-DDH Digital - DNA DNA hybridization -- ANI Average nucleotide identity -- IAA Indole acetic acid -- PGPA Plant growth promoting actinobacteria -- C. tagal Ceriops tagal -- TSA Tryptic soy agar -- WGS Whole genome sequencing -- PGP Plant growth promotion; Agricultural biotechnology -- Periodicals; Enzymes -- Biotechnology -- Periodicals; 660.6
• DOI: 10.1016/j.bcab.2022.102543
• Languages: English
• ISSNs: 1878-8181

Abstract

Abstract: Reports on Sporosarcina species associated with mangroves native plants and their diversity are scarced. Draft genome of Sporosarcina sp. strain NCCP-2222 T, isolated from the rhizosphere of Ceriops tagal, a mangrove plant from the Lasbela, Pakistan, is presented. The assembled genome of NCCP-2222 T features genome size of 4, 019, 411 bp, DNA G+C content of 44.4%, and predicted 3990 protein coding genes. The digi-DDH and ANI analyses indicated that strain NCCP-2222 T represents a novel species in genus Sporosarcina . Genome mining disclosed the presence of genes involved in solubilization of phosphorus and production of indole acetic acid (IAA) in strain NCCP-2222 T . Draft genome of Sporosarcina sp. NCCP-2222 T reveals insights on its potential as a plant biostimulant and provides evidence to represent it as a novel species, expanding our knowledge regarding Sporosarcina biodiversity worldwide and specifically those strains associated with native mangroves plants in Pakistan. Highlights: The digi-DDH and ANI analyses indicate that strain NCCP-2222 T represents a novel species in genus Sporosarcina. Genome mining disclose the presence of genes involved in solubilization of phosphorus and IAA production in strain NCCP-2222 T, which showed its potentials as plant-biostimulant and can be used in development of biofertilizers. Sporosarcina sp. NCCP-2222 T is the first novel species being reported from the mangrove ecology of Pakistan.