Improvement of selenium enrichment in Rhodotorula glutinis X-20 through combining process optimization and selenium transport. Issue 1 (1st January 2019)

• Record Type: Journal Article
• Authors: Wang, Ting; Lou, Xindan; Zhang, Genlin; Dang, Yanyan
• Is Part Of: Bioengineered. Volume 10:Issue 1(2019)
• Page Start: 335
• Page End: 344
• Publication Date: 2019-01-01
• Subjects: Selenium-enriched yeast -- mixed carbon -- enrichment -- transport -- R. glutinis -- culture optimization; Biomedical engineering -- Periodicals; Biotechnology -- Periodicals; Microbiology -- Periodicals; 660.6
• DOI: 10.1080/21655979.2019.1644853
• Languages: English
• ISSNs: 2165-5987

Abstract

ABSTRACT: Selenium-enriched yeast can transform toxic inorganic selenium into absorbable organic selenium, which is of great significance for human health and pharmaceutical industry. A yeast Rhodotorula glutinis X-20 we obtained before has good selenium-enriched ability, but its selenium content is still low for industrial application. In this study, strategies of process optimization and transport regulation of selenium were thus employed to further improve the cell growth and selenium enrichment. Through engineering phosphate transporters from Saccharomyces cerevisiae into R. glutinis X-20, the selenium content was increased by 21.1%. Through using mixed carbon culture (20 g L −1, glycerol: glucose 3:7), both biomass and selenium content were finally increased to 5.3 g L −1 and 5349.6 µg g −1 (cell dry weight, DWC), which were 1.14 folds and 6.77 folds compared to their original values, respectively. Our results indicate that high selenium-enrichment ability and biomass production can be achieved through combining process optimization and regulation of selenium transport. Graphical abstract: uf0001