Evaluation of the anti-oxidative and ROS scavenging properties of biomaterials coated with epigallocatechin gallate for tissue engineering. (1st April 2021)

• Record Type: Journal Article
• Authors: Lee, Sangmin; Lee, Jinkyu; Byun, Hayeon; Kim, Se-jeong; Joo, Jinmyoung; Park, Hee Ho; shin, Heungsoo
• Is Part Of: Acta biomaterialia. Volume 124(2021)
• Page Start: 166
• Page End: 178
• Publication Date: 2021-04-01
• Subjects: Tissue engineering -- Oxidative stress -- Reactive oxygen species -- Antioxidant -- Polyphenol coating; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1016/j.actbio.2021.02.005
• Languages: English
• ISSNs: 1742-7061

Abstract

Abstract: In tissue engineering, excessively generated reactive oxygen species (ROS) during biomaterial implantation or cell transplantation is a one of major causes of diminishing therapeutic effects. In this study, we prepared biomaterial surfaces coated with antioxidant epigallocatechin gallate (EGCG) and metal ions, and evaluated their anti-oxidative and ROS scavenging properties. We revealed that EGCG-coating on polycaprolactone (PCL) film surface increased hydrophilicity and anti-oxidative properties as a function of total phenol content (TPC) potentially due to the increase in phenolic -OH and π-electrons from structural maintenance and directly removed the hydrogen peroxide (H2 O2 ) by resonance-stabilization. Furthermore, EGCG-coated PCL film increased attachment, spreading area, and viability of human adipose-derived stem cells (hADSCs) against H2 O2 treatment while stimulated the cellular signaling to reduce apoptotic gene and enhance anti-oxidative enzyme expression. Further, we applied EGCG coating on the surface of poly-L-lactic acid (PLLA) fibers. Spheroids incorporating EGCG-coated PLLA fibers were able to maintain their shape and showed improved viability and anti-oxidative activities in response to H2 O2 -induced oxidative stress than control spheroids. Therefore, metal-phenolic network (MPN) coating of EGCG is a suitable method to impart the anti-oxidative properties to biomaterials by evaluating the structural properties and biological effects. Graphic abstract: Image, graphical abstract