Nanoscopic evaluation on mitochondrial ultrastructures by regulating reactive oxygen species productivity within terpyridyl Zn(ii) complexes with different alkyl chain lengths. Issue 1 (12th December 2022)

• Record Type: Journal Article
• Authors: Su, Liping; Xian, Jinghong; Fu, Shiqin; Zhu, Yuhan; Cao, Hongzhi; Feng, Zhihui; Tian, Yupeng; Tian, Xiaohe
• Is Part Of: Nanoscale. Volume 15:Issue 1(2023)
• Page Start: 350
• Page End: 355
• Publication Date: 2022-12-12
• Subjects: Nanoscience -- Periodicals; Nanotechnology -- Periodicals; 620.505
• DOI: 10.1039/d2nr04088c
• Languages: English
• ISSNs: 2040-3364

Abstract

Abstract : Scheme capturing the ultrastructural morphology of mitochondria by regulating ROS productivity within terpyridyl Zn(ii ) complexes with different alkyl chain lengths. Mitochondrial (image ID YUOYPde40d) images were drawn using Figdraw. Abstract : Mitochondria targeting complexes are widely utilized as photosensitizers in photodynamic therapy. However, the mechanisms by which they regulate reactive oxygen species (ROS) production at the molecular level and their influence on intracellular mitochondrial signaling and ultrastructures remain rarely studied. Herein, we present two terpyridyl Zn(ii ) complexes with different side alkyl chain lengths (Zn–2C and Zn–6C) that lead to low and high ROS productivities in vitro, respectively. Both complexes could enter live cells effectively with minimal dark toxicity and accumulate preferably in the mitochondria. We also demonstrated that Zn–6C, with more efficient ROS productivity, could significantly downregulate the caspase signaling pathway but showed no evident influence on mitochondrial membrane proteins. We also highlighted and compared the mitochondrial ultrastructural variations during such a process by stimulated emission depletion (STED) super-resolution nanoscopy.