The pluripotency transcription factor OCT4 represses heme oxygenase‐1 gene expression. Issue 14 (28th June 2021)

• Record Type: Journal Article
• Authors: Petrone, María Victoria; Toro, Ayelén; Vazquez Echegaray, Camila; Francia, Marcos Gabriel; Solari, Claudia; Cosentino, Maria Soledad; Vazquez, Elba; Guberman, Alejandra
• Editors: Mantei, Ned
• Is Part Of: FEBS letters. Volume 595:Issue 14(2021)
• Page Start: 1949
• Page End: 1961
• Publication Date: 2021-06-28
• Subjects: gene modulation -- heme oxygenase‐1 -- OCT4 -- pluripotency transcription factors -- pluripotent stem cells -- transcription; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1002/1873-3468.14138
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : In embryonic stem (ES) cells, oxidative stress control is crucial for genomic stability, self‐renewal, and cell differentiation. Heme oxygenase‐1 (HO‐1) is a key player of the antioxidant system and is also involved in stem cell differentiation and pluripotency acquisition. We found that the HO‐1 gene is expressed in ES cells and induced after promoting differentiation. Moreover, downregulation of the pluripotency transcription factor (TF) OCT4 increased HO‐1 mRNA levels in ES cells, and analysis of ChIP‐seq public data revealed that this TF binds to the HO‐1 gene locus in pluripotent cells. Finally, ectopic expression of OCT4 in heterologous systems repressed a reporter carrying the HO‐1 gene promoter and the endogenous gene. Hence, this work highlights the connection between pluripotency and redox homeostasis. Abstract :