Distinct microbiota assembly mechanisms revealed in different reconstruction stages during gut regeneration in the sea cucumber Apostichopus japonicus. Issue 6 (22nd November 2021)

• Record Type: Journal Article
• Authors: Yu, Zichao; Xue, Zhuang; Liu, Chao; Zhang, Anguo; Fu, Qiang; Yang, Kun; Zhang, Fang; Ran, Liyuan
• Is Part Of: MicrobiologyOpen. Volume 10:Issue 6(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-11-22
• Subjects: Apostichopus japonicus -- evisceration -- gut microbiota reconstruction -- gut regeneration; Microbiology -- Periodicals; 579
• DOI: 10.1002/mbo3.1250
• Languages: English
• ISSNs: 2045-8827

Abstract

Abstract: Apostichopus japonicus is a useful model for studying organ regeneration, and the gut microbiota is important for host organ regeneration. However, the reconstruction process and the mechanisms of gut microbiota assembly during gut regeneration in sea cucumbers have not been well studied. In the present study, gut regeneration was induced (via evisceration) in A . japonicus, and gut immune responses and bacterial diversity were investigated to reveal gut microbiota assembly and its possible mechanisms during gut regeneration. The results revealed that bacterial community reconstruction involved two stages with distinct assembly mechanisms, where the reconstructed community was initiated from the bacterial consortium in the residual digestive tract and tended to form a novel microbiota in the later stage of reconstruction. Together, the results of immunoenzyme assays, community phylogenetic analysis, and source tracking suggested that the host deterministic process was stronger in the initial stage than in the later stage. The bacterial interactions that occurred were significantly different between the two stages. Positive interactions dominated in the initial stage, while more complex and competitive interactions developed in the later stage. Such a dynamic bacterial community could provide the host with energetic and immune benefits that promote gut regeneration and functional recovery. The results of the present study provide insights into the processes and mechanisms of gut microbiota assembly during intestinal regeneration that are valuable for understanding gut regeneration mechanisms mediated by the microbiota. Abstract : Ejection of internal organs was induced in the sea cucumber Apostichopus japonicus, and gut immune responses and bacterial diversity during gut regeneration were investigated. We revealed that the reconstruction of the bacterial community involved two stages. The diversity, taxonomic composition, and bacterial interaction of the gut bacterial community and the interaction between the bacterial community and the host were significantly different in the two stages.