Evaluation of the early developmental neural toxicity of F-53B, as compared to PFOS, with an in vitro mouse stem cell differentiation model. (August 2018)

• Record Type: Journal Article
• Authors: Yin, Nuoya; Yang, Renjun; Liang, Shaojun; Liang, Shengxian; Hu, Bowen; Ruan, Ting; Faiola, Francesco
• Is Part Of: Chemosphere. Volume 204(2018)
• Page Start: 109
• Page End: 118
• Publication Date: 2018-08
• Subjects: F-53B -- Perfluorooctane sulfonate (PFOS) -- Developmental neural toxicity -- Embryonic stem cells (ESCs) -- Neural differentiation -- Neuroectoderm; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2018.04.011
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: F-53B, as an alternative to the persistent organic pollutant perfluorooctane sulfonate (PFOS), is amply used in the electric plating industry. F-53B and PFOS have similar physicochemical, biochemical and physiological properties, due to the similarity in their chemical structure. Thus, they may also possess similar toxicities. Although epidemiological studies and in vivo assays have shown that prenatal exposure to PFOS may impair the development of the nervous system, toxicity data for F-53B are still scarce. In this study, we employed an embryonic stem cell (ESC) in vitro differentiation system, to detect the potential developmental neural toxicity of F-53B and PFOS, at human exposure relevant doses. We demonstrated that during early mouse ESC (mESC) neural differentiation, F-53B and PFOS disrupted the expression of neural marker genes and affected the morphology of the differentiated cells. However, the very same treatments did not cause any cytotoxic effects. In conclusion, our ESC in vitro differentiation system was able to prove for the first time that F-53B and PFOS at human exposure relevant concentrations, could alter the expression of differentiation biomarkers, indicating a potential developmental neural toxicity. Based on our findings, it is reasonable to deduce that excessive exposure to F-53B and PFOS may cause severe dysfunctions during early stages of embryo development. Highlights: F-53B and PFOS developmental toxicity was assessed with mouse embryonic stem cells. Human relevant F-53B and PFOS doses may cause neural toxicity early in development. F-53B and PFOS impair the specification of MAP2 positive neural progenitor cells.