Diallyl trisulphide inhibits adipogenesis in 3T3-L1 adipocytes through lipogenesis, fatty acid transport, and fatty acid oxidation pathways. (June 2015)

• Record Type: Journal Article
• Authors: Chang, Wei-Tang; Wu, Chi-Hao; Hsu, Chin-Lin
• Is Part Of: Journal of functional foods. Volume 16(2015)
• Page Start: 414
• Page End: 422
• Publication Date: 2015-06
• Subjects: ACC acetyl-CoA carboxylase -- alliin S-Allyl-L-cysteine sulphoxide -- aP2 adipocyte fatty acid-binding protein 2 -- C/EBPα CCAAT enhancer binding protein alpha -- DAS diallyl sulphide -- DADS diallyl disulphide -- DATS diallyl trisulphide -- DEX dexamethasone -- DMEM Dulbecco's modified Eagle's medium -- DMSO dimethyl sulphoxide -- FAS fatty acid synthase -- GPDH glycerol-3-phosphate dehydrogenase -- IBMX 3-isobutyl-1-methylxanthine -- INS insulin -- LPL lipoprotein lipase -- MCP-1 monocyte chemoattractant protein-1 -- OROSM oil red O-stained material -- OSC organo-sulphur compounds -- PAI-1 plasminogen activator inhibitor-1 -- PGC-1β peroxisome proliferator-activated receptor-coactivator 1β -- PPARγ peroxisome proliferator-activated receptor-gamma -- SAC S-allylcysteine -- UCP1 uncoupling protein 1; Diallyl trisulphide -- Adipogenesis -- 3T3-L1 adipocytes -- Lipogenesis -- Fatty acid oxidation; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2015.05.002
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: DATS decreases intracellular triglyceride and GPDH activity in 3T3-L1 adipocytes. DATS increases adiponectin production in 3T3-L1 adipocytes. DATS inhibits adipogenesis in 3T3-L1 adipocytes. DATS regulates gene levels of lipogenesis, lipolysis, and adipokines. Abstract: Garlic and its related products (such as black garlic) exhibit multiple biological functions and attributed to the presence of organo-sulphur compounds. The aim of this study was to investigate the inhibitory effects of garlic-derived organo-sulphur compounds [including diallyl sulphide (DAS), diallyl disulphide (DADS), diallyl trisulphide (DATS), S-allylcysteine (SAC), and S-allyl-L-cysteine sulphoxide (alliin)] on adipogenesis in 3T3-L1 adipocytes. The result indicated that DATS significantly decreased the intracellular triacylglycerol, oil red O-stained material (OROSM), and glycerol-3-phosphate dehydrogenase (GPDH) activity and significantly increased the adiponectin production in 3T3-L1 adipocytes. Real-time RT-PCR analysis showed that DATS decreased the gene levels of adipogenesis (C/EBPα, PPARγ, ACC, and FAS), fatty acid transport (aP2, CD36, and LPL), and adipocytokines (leptin, MCP-1, PAI-1, and resistin), and increased the gene levels of fatty acid oxidation (PGC-1β and UCP1) and adiponectin in 3T3-L1 adipocytes. DATS is capable of inhibiting adipogenesis in 3T3-L1 adipocytes possibly through regulation of gene levels of lipogenesis, lipolysis, and adipokines.