Telomerase Reverse Transcriptase Expression Marks a Population of Rare Adipose Tissue Stem Cells. Issue 1 (19th January 2022)

• Record Type: Journal Article
• Authors: Lynes, Matthew D; Carlone, Diana L; Townsend, Kristy L; Breault, David T; Tseng, Yu-Hua
• Is Part Of: Stem cells. Volume 40:Issue 1(2022)
• Page Start: 102
• Page End: 111
• Publication Date: 2022-01-19
• Subjects: adipose tissue -- telomerase -- adipogenesis -- adult stem cells; Cloning -- Periodicals; Clone cells -- Periodicals; Stem cells -- Periodicals; Cell Differentiation -- Periodicals; Cell Division -- Periodicals; Clone Cells -- Periodicals; Hematopoietic Stem Cells -- Periodicals; Stem Cells -- Periodicals; 571.84
• DOI: 10.1093/stmcls/sxab005
• Languages: English
• ISSNs: 1066-5099

Abstract

Abstract: In adult tissues such as adipose tissue, post-mitotic cells like adipocytes can be replaced by differentiation of a population of tissue-resident stem cells. Expression of mouse telomerase reverse transcriptase (mTert) is a hallmark of stem cell populations, and previous efforts to identify tissue-resident adult stem cells by measuring mTert expression have increased our understanding of stem cell biology significantly. Here, we used a doxycycline-inducible mouse model to perform longitudinal, live-animal lineage-tracing of mTert-expressing cells for more than 1 year. We identified a rare (<2%) population of stem cells in different fat depots that express putative preadipocyte markers. The adipose-derived mTert-positive cells are capable of self-renewal and possess adipogenic potential. Finally, we demonstrate that high-fat diet (HFD) can initiate differentiation of these cells in vivo. These data identify a population of adipose stem cells that contribute to the depot-specific response to HFD. Graphical Abstract: