Cisplatin-functionalized three-dimensional magnetic SBA-16 for treating breast cancer cells (MCF-7). Issue 1 (4th December 2019)

• Record Type: Journal Article
• Authors: Alomari, Munther; Jermy, B. Rabindran; Ravinayagam, Vijaya; Akhtar, Sultan; Almofty, Sarah Ameen; Rehman, Suriya; Bahmdan, Hiba; AbdulAzeez, Sayed; Borgio, J. Francis
• Is Part Of: Artificial cells, nanomedicine, and biotechnology. Volume 47:Issue 1(2019)
• Page Start: 3079
• Page End: 3086
• Publication Date: 2019-12-04
• Subjects: SPIONs -- cisplatin -- SBA-16 -- polyacrylic acid -- nanotherapeutics -- cancer therapy -- MCF-7 -- HFF-1 -- TEM; Artificial cells -- Periodicals; Nanotechnology -- Periodicals; Blood substitutes -- Periodicals; Tissue engineering -- Periodicals; Molecules -- Periodicals; Biotechnology -- Periodicals; 615.39
• DOI: 10.1080/21691401.2019.1645155
• Languages: English
• ISSNs: 2169-1401

Abstract

Abstract: The engineering of multifunctional therapeutics in an integrated single platform is demonstrated using three-dimensional SBA-16 (S-16). 10 wt% iron oxide nanoparticles (Fe) were loaded into the cage type of cubic pores through enforced adsorption technique. Fe/S-16 is then functionalized with amine-based silane (A), polyacrylic acid (P) and cisplatin (Cp). The physicochemical textural analysis showed the formation of nano metal oxide distributions at pore walls of S-16 with magnetization of 2.39 emu/g. S-16 based nanoformulations showed high percentage of Cp adsorption (90%) and percentage cumulative release (60%). in vitro study of Fe/S-16-A-Cp showed high toxicity against breast cancer cell line MCF-7 and normal cell line Human foreskin fibroblast (HFF-1) compared to Fe/S-16 indicating cisplatin profusion inside the cells than free cisplatin. While skin fibroblast seems to be resistant to Fe/S-16-AP-Cp with very high LC50 in compare to MCF-7. This indicates the unrelease of cisplatin in skin fibroblast after Fe/S-16-AP-Cp treatment due to effective encapsulation inside the cubic pores and core blockage due to pH-sensitive polyacrylic acid. Also, these treatments resulted in morphological changes in the cells such as DNA condensation and nuclear fragmentation.