Bioactive flavonoids from plant extract of Pyrethrum pulchrum and its acute toxicity. Issue 24 (17th December 2021)

• Record Type: Journal Article
• Authors: Erdenetsogt, Uugangerel; Nadmid, Suvd; Paulus, Constanze; Chanagsuren, Ganchimeg; Dolgor, Erdenechimeg; Gotov, Choijamts; Dahse, Hans-Martin; Luzhetskyy, Andriy; Dagvadorj, Enkhmaa
• Is Part Of: Natural product research. Volume 35:Issue 24(2021)
• Page Start: 5960
• Page End: 5963
• Publication Date: 2021-12-17
• Subjects: Pyrethrum pulchrum -- Asteraceae (Compositae) -- flavonoid -- antiproliferative activity -- cytotoxicity; Natural products -- Periodicals; Natural products -- Research -- Periodicals; Biological Factors -- Periodicals; Biological Products -- Periodicals; 547
• DOI: 10.1080/14786419.2020.1806271
• Languages: English
• ISSNs: 1478-6419

Abstract

Abstract: Pyrethrum pulchrum Ledeb. has been a phytochemically unexplored Mongolian medicinal folklore plant. In this study, its total flavonoid content was determined and fourteen flavonoids (1-14 ) were isolated from the aerial parts of P. pulchrum . Their structures were elucidated on the basis of spectroscopic data. The compounds 12 -14, methoxyflavones, were tested for antiproliferative and cytotoxic activity against A549, HeLa, K-562, THP-1 and HUVEC cell lines. This is the first report on the effects of 5, 7, 4'-trihydroxy-3, 6, 3'-trimethoxyflavone (13 ) against all tested cell lines and it exhibited potent activity against chronic myeloid leukemia K-562 and acute monocytic leukemia THP-1 cells, each with GI50 value at 2.0 μg/mL. The 5, 4'-dihydroxy-3, 6, 7, 3'-tetramethoxyflavone (14 ) showed the most potent activity against THP-1 (GI50 = 1.1 μg/mL) and the highest cytotoxicity (5.6 μg/mL). In addition, acute toxicity of plant ethanol extract was evaluated and the lethal dose (LD50 ) was estimated at 1048 mg/kg. Graphical Abstract: UF0001