Characterization of synergistic antibacterial effect of silver nanoparticles and ebselen. Issue 1 (4th December 2019)

• Record Type: Journal Article
• Authors: Chen, Xueqing; Chen, Heming; Zhang, Hongying; Peng, Yanjuan; Deng, Fuchang; Gao, Jiye; Chai, Chunli; Tang, Song; Zuo, Xin; Lu, Jun; Du, Huamao
• Is Part Of: Artificial cells, nanomedicine, and biotechnology. Volume 47:Issue 1(2019)
• Page Start: 3338
• Page End: 3349
• Publication Date: 2019-12-04
• Subjects: Silver nanoparticles -- ebselen -- multi-drug resistance bacteria -- synergistic antibacterial effect -- oxidative stress -- thioredoxin reductase; Artificial cells -- Periodicals; Nanotechnology -- Periodicals; Blood substitutes -- Periodicals; Tissue engineering -- Periodicals; Molecules -- Periodicals; Biotechnology -- Periodicals; 615.39
• DOI: 10.1080/21691401.2019.1648278
• Languages: English
• ISSNs: 2169-1401

Abstract

Abstract: The emerging and spreading of multi-drug resistant (MDR) bacteria have been becoming one of the most severe threats to human health. Enhancing oxidative stress as mimicking immune system was considered as a potential strategy to fight against infection of MDR bacteria. In this study, we investigated the antibacterial efficiency of such a strategy which combines silver nanoparticles (AgNPs) with ebselen. The results showed that AgNPs and ebselen combination had significant synergistic killing effects both on Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ) in vitro, including model strains of China Veterinary Culture Collection and MDR clinical isolates, which is similar as the combination of silver ion and ebselen. AgNPs exhibited to be a strong inhibitor of bacterial thioredoxin reductase, same as a free silver ion. Ebselen mitigated the cytotoxicity of AgNPs to HeLa cells. However, in a bacteria-cell coexistence condition, the synergistic bactericidal effect was only observed on S. aureus ( p <.05), while the temporary synergistic inhibitory effect on E. coli within 4 hours treatment ( p <.01). In mice infection model, a combination of AgNPs and ebselen did not increase protection against the challenge of clinical E. coli CQ10 strain. Our data demonstrated that AgNPs and ebselen combination may be a promising strategy to fight against the increasingly MDR bacteria targeting bacterial thiol redox system.