Development of HANABI, an ultrasonication-forced amyloid fibril inducer. (February 2022)

• Record Type: Journal Article
• Authors: Goto, Yuji; Nakajima, Kichitaro; Yamaguchi, Keiichi; So, Masatomo; Ikenaka, Kensuke; Mochizuki, Hideki; Ogi, Hirotsugu
• Is Part Of: Neurochemistry international. Volume 153(2022)
• Publication Date: 2022-02
• Subjects: α-synuclein -- β2-microglobulin -- Amyloid fibril -- Solubility -- Supersaturation -- Ultrasonication; Neurochemistry -- Periodicals; Neurochemistry -- Periodicals; Neurochimie -- Périodiques; Neurochemistry; Periodicals; 612.804205
• DOI: 10.1016/j.neuint.2021.105270
• Languages: English
• ISSNs: 0197-0186

Abstract

Abstract: Amyloid fibrils involved in amyloidoses are crystal-like aggregates, which are formed by breaking supersaturation of denatured proteins. Ultrasonication is an efficient method of agitation for breaking supersaturation and thus inducing amyloid fibrils. By combining an ultrasonicator and a microplate reader, we developed the HANABI (HANdai Amyloid Burst Inducer) system that enables high-throughput analysis of amyloid fibril formation. Among high-throughput approaches of amyloid fibril assays, the HANABI system has advantages in accelerating and detecting spontaneous amyloid fibril formation. HANABI is also powerful for amplifying a tiny amount of preformed amyloid fibrils by seeding. Thus, HANABI will contribute to creating therapeutic strategies against amyloidoses by identifying their biomarkers. Highlights: Amyloid fibrils are crystal-like aggregates formed by breaking supersaturation. Ultrasonication breaks supersaturation and induces amyloid fibrils. HANABI enables high-throughput analysis of amyloid fibril formation. HANABI will be powerful for identifying susceptibility risk biomarkers.