Polymerization-sensitive switch-on monomer for terminal transferase activity assay. Issue 1 (4th December 2019)

• Record Type: Journal Article
• Authors: Batule, Bhagwan S.; Lee, Chang Yeol; Park, Ki Soo; Park, Hyun Gyu
• Is Part Of: Artificial cells, nanomedicine, and biotechnology. Volume 47:Issue 1(2019)
• Page Start: 256
• Page End: 259
• Publication Date: 2019-12-04
• Subjects: Terminal transferase -- BODIPY-ATP -- Fe(III) -- Fluorescent sensor -- Enzyme activity; Artificial cells -- Periodicals; Nanotechnology -- Periodicals; Blood substitutes -- Periodicals; Tissue engineering -- Periodicals; Molecules -- Periodicals; Biotechnology -- Periodicals; 615.39
• DOI: 10.1080/21691401.2018.1552593
• Languages: English
• ISSNs: 2169-1401

Abstract

Abstract: We herein describe a simple but efficient method for the determination of terminal transferase (TdT) activity, which relies on our finding that Fe(III)-quenched boron-dipyrromethene (BODIPY)-ATP is utilized as a switch-on monomer for polymerization and enables the facile synthesis of fluorescence oligonucleotides without additional, post-processing steps. As TdT carries out the synthesis of DNA by adding the monomers into growing chains, Fe(III) is displaced from BODIPY with the release of pyrophosphate group, which consequently leads to the generation of highly fluorescent long oligonucleotides. With this strategy, we selectively detected the TdT activity with high sensitivity. In addition, its practical applicability was successfully demonstrated by determining TdT activities in human serum.