Swarming Magnetic Microrobots for Pathogen Isolation from Milk. Issue 6 (7th December 2022)

• Record Type: Journal Article
• Authors: Mayorga‐Martinez, Carmen C.; Castoralova, Marketa; Zelenka, Jaroslav; Ruml, Tomas; Pumera, Martin
• Is Part Of: Small. Volume 19:Issue 6(2023)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-12-07
• Subjects: bacteria -- collective behavior -- infections -- micromachines; Nanotechnology -- Periodicals; Nanoparticles -- Periodicals; Microtechnology -- Periodicals; 620.5
• DOI: 10.1002/smll.202205047
• Languages: English
• ISSNs: 1613-6810

Abstract

Abstract: Bovine mastitis produced by Staphylococcus aureus ( S. aureus ) causes major problems in milk production due to the staphylococcal enterotoxins produced by this bacterium. These enterotoxins are stable and cannot be eradicated easily by common hygienic procedures once they are formed in dairy products. Here, magnetic microrobots (MagRobots) are developed based on paramagnetic hybrid microstructures loaded with IgG from rabbit serum that can bind and isolate S. aureus from milk in a concentration of 3.42 10 4 CFU g −1 (allowable minimum level established by the United States Food and Drug Administration, FDA). Protein A, which is present on the cell wall of S. aureus, selectively binds IgG from rabbit serum and loads the bacteria onto the surface of the MagRobots. The selective isolation of S. aureus is confirmed using a mixed suspension of S. aureus and Escherichia coli ( E. coli ). Moreover, this fuel‐free system based on magnetic robots does not affect the natural milk microbiota or add any toxic compound resulting from fuel catalysis. This system can be used to isolate and transport efficiently S. aureus and discriminate it from nontarget bacteria for subsequent identification. Finally, this system can be scaled up for industrial use in food production. Abstract : Magnetic microrobots (MagRobots) based on paramagnetic hybrid microstructures modified with anti‐rabbit IgG produced in goat and IgG from rabbit serum, that can specifically bind and isolate S. aureus bacteria from milk using the protein A present on the bacteria cell wall for efficient bacteria removal as well as their specific determination.