Engineered Phage with Aggregation‐Induced Emission Photosensitizer in Cocktail Therapy against Sepsis. Issue 6 (19th December 2022)

• Record Type: Journal Article
• Authors: Wu, Ming‐Yu; Chen, Luojia; Chen, Qingrong; Hu, Rui; Xu, Xiaoyu; Wang, Yifei; Li, Jie; Feng, Shun; Dong, Changjiang; Zhang, Xiao‐Lian; Li, Zhiqiang; Wang, Lianrong; Chen, Shi; Gu, Meijia
• Is Part Of: Advanced materials. Volume 35:Issue 6(2023)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-12-19
• Subjects: aggregation‐induced emission -- phage cocktail therapy -- photodynamic therapy -- photosensitizers -- sepsis; Materials -- Periodicals; Chemical vapor deposition -- Periodicals; 620.11
• DOI: 10.1002/adma.202208578
• Languages: English
• ISSNs: 0935-9648

Abstract

Abstract: Sepsis, a widely recognized disease, is characterized by multiple pathogen infections. Therefore, it is imperative to develop methods that can efficiently identify and neutralize pathogen species. Phage cocktail therapy utilizes the host specificity of phages to adapt to infect resistant bacteria. However, its low sterilization stability efficiency and lack of imaging units seriously restrict its application. Here, a novel strategy combining the aggregation‐induced emission photosensitizer (AIE‐PS) TBTCP‐PMB with phages through a nucleophilic substitution reaction between benzyl bromide and sulfhydryl groups to remove pathogenic bacteria for sepsis treatment is proposed. This strategy retains the phage's host specificity while possessing AIE‐PS characteristics with a fluorescence imaging function and reactive oxygen species (ROS) for detecting and sterilizing bacteria. This synergetic strategy combining phage cocktail therapy and photodynamic therapy (PDT) shows a strong "1 + 1 > 2" bactericidal efficacy and superior performance in sepsis mouse models with good biocompatibility. Furthermore, the strategy can quickly diagnose blood infections of clinical blood samples. This simple and accurate strategy provides a promising therapeutic platform for rapid pathogen detection and point‐of‐care diagnosis. Moreover, it presents a new method for expanding the library of antibacterial drugs to develop new strain identification and improve infectious disease treatment, thereby demonstrating strong translational potential. Abstract : A novel strategy combining the aggregation‐induced emission photosensitizer with phages is developed that not only selectively identifies bacterial species but also shows a powerful ability to kill pathogenic bacteria in sepsis diagnosis and treatment. This synergetic therapy strategy combining phage cocktail therapy and photodynamic therapy offers a strong "1 + 1 > 2" bactericidal efficacy in vitro and in vivo.