Investigating the oxidase-like activity of a Co–Fe Prussian blue analogue nanocube prepared in situ and its applications in the colorimetric detection of ascorbic acid, alkaline phosphatase, α-glucosidase, and ascorbic acid oxidase. (13th December 2022)

• Record Type: Journal Article
• Authors: Peng, Li-Jing; Yin, Shi-Jun; Chen, Li; Tian, Tao; Zhang, Wei-Yi; Zhou, Hang-Yu; Yang, Feng-Qing
• Is Part Of: New journal of chemistry. Volume 47:Number 3(2023)
• Page Start: 1156
• Page End: 1164
• Publication Date: 2022-12-13
• Subjects: Chemistry -- Periodicals; Chimie -- Périodiques; 540
• DOI: 10.1039/d2nj05460d
• Languages: English
• ISSNs: 1144-0546

Abstract

Abstract : A Co–Fe Prussian blue analogue nanocube (Co–Fe PBA NC) was prepared in situ . Based on the excellent oxidase-like activity of Co–Fe PBA NC, a colorimetric sensor is developed for the detection of ascorbic acid-related bioenzymes. Abstract : Herein, a Co–Fe Prussian blue analogue nanocube (Co–Fe PBA NC) was prepared in situ via a simple and rapid coprecipitation method at room temperature, and the synthesized nanocube was systematically characterized. The Co–Fe PBA NC was proven to have intrinsic oxidase-like activity, which can catalyze the production of reactive oxygen species, such as superoxide anions (O2 ˙ − ), hydroxyl radicals (˙OH), and singlet oxygen ( 1 O2 ), in a dissolved oxygen system without the presence of hydrogen peroxide (H2 O2 ). By making full use of the oxidase-like activity of the Co–Fe PBA NC and using the chromogenic substrate 3, 3′, 5, 5′-tetramethylbenzidine (TMB), a colorimetric sensor was fabricated for the detection of ascorbic acid (AA) and its related biological enzymes' activities, including alkaline phosphatase (ALP), α-glucosidase (α-Glu), and ascorbic acid oxidase (AAO), in human serum. This study presents an oxidase mimic that can be synthesized via a simple, rapid, and low-cost method, which overcomes the defects of cumbersome and time-consuming synthetic process, dependence on professional instruments and high cost of some previously reported nanozymes. In addition, this study provides a new multifunctional colorimetric sensing platform for AA and its related biological enzymes, showing great potential in biological assays.