MicroRNA-495 induces breast cancer cell migration by targeting JAM-A. Issue 11 (30th July 2014)

• Record Type: Journal Article
• Authors: Cao, Minghui; Nie, Weiwei; Li, Jing; Zhang, Yujing; Yan, Xin; Guan, Xiaoxiang; Chen, Xi; Zen, Ke; Zhang, Chen-yu; Jiang, Xiaohong; Hou, Dongxia
• Is Part Of: Protein & cell. Volume 5:Issue 11(2014)
• Page Start: 862
• Page End: 872
• Publication Date: 2014-07-30
• Subjects: miR-495 -- JAM-A -- breast cancer -- migration; Proteins -- Periodicals; Cells -- Periodicals; Cytology -- Periodicals; 572.6
• DOI: 10.1007/s13238-014-0088-2
• Languages: English
• ISSNs: 1674-800X

Abstract

Abstract: MicroRNAs (miRNAs) are small, non-coding RNAs that function as post-transcriptional regulators of gene expression. The deregulated expression of miRNAs is associated with a variety of diseases, including breast cancer. In the present study, we found that miR-495 was markedly up-regulated in clinical breast cancer samples by quantitative real time-PCR (qRT-PCR). Junctional adhesion molecule A (JAM-A) was predicted to be a potential target of miR-495 by bioinformatics analysis and was subsequently verified by luciferase assay and Western blotting. JAM-A was found to be negatively correlated with the migration of breast cancer cells through loss-of-function and gain-of-function assays, and the inhibition of JAM-A by miR-495 promoted the migration of MCF-7 and MDA-MB-231 cells. Furthermore, overexpression of JAM-A could restore miR-495-induced breast cancer cell migration. Taken together, our findings suggest that miR-495 could facilitate breast cancer progression through the repression of JAM-A, making this miRNA a potential therapeutic target.