Applications of RNA interference high-throughput screening technology in cancer biology and virology. Issue 11 (22nd June 2014)

• Record Type: Journal Article
• Authors: Gao, Shan; Yang, Chen; Jiang, Shan; Xu, Xiao-Ning; Lu, Xin; He, You-Wen; Cheung, Annie; Wang, Hui
• Is Part Of: Protein & cell. Volume 5:Issue 11(2014)
• Page Start: 805
• Page End: 815
• Publication Date: 2014-06-22
• Subjects: RNA interference (RNAi) -- short interfering RNA (siRNA) -- short hairpin RNA (shRNA) -- high-throughput screening -- cancer -- virology; Proteins -- Periodicals; Cells -- Periodicals; Cytology -- Periodicals; 572.6
• DOI: 10.1007/s13238-014-0076-6
• Languages: English
• ISSNs: 1674-800X

Abstract

Abstract: RNA interference (RNAi) is an ancient intra-cellular mechanism that regulates gene expression and cell function. Large-scale gene silencing using RNAi high-throughput screening (HTS) has opened an exciting frontier to systematically study gene function in mammalian cells. This approach enables researchers to identify gene function in a given biological context and will provide considerable novel insight. Here, we review RNAi HTS strategies and applications using case studies in cancer biology and virology.