A biotin-guided near-infrared fluorescent probe for imaging hydrogen sulfide and differentiating cancer cells. Issue 2 (19th December 2022)

• Record Type: Journal Article
• Authors: Cai, Xuekang; Zhang, Zhuochen; Dong, Yalun; Hao, Tingting; Yi, Long; Yang, Xing
• Is Part Of: Organic & biomolecular chemistry. Volume 21:Issue 2(2023)
• Page Start: 332
• Page End: 338
• Publication Date: 2022-12-19
• Subjects: Chemistry, Organic -- Periodicals; Bioorganic chemistry -- Periodicals; Chemistry, Physical organic -- Periodicals; 547
• DOI: 10.1039/d2ob02034c
• Languages: English
• ISSNs: 1477-0520

Abstract

Abstract : A biotin-guided near-infrared fluorescent probe could be transported into cancer cells selectively and could further detect H2 S. Abstract : Imaging cancer specific biomarkers with near-infrared (NIR) fluorescent probes can help inaccurate diagnosis. Hydrogen sulfide (H2 S) has been reported to be involved in many physiological and pathological processes and is considered as one of the key gasotransmitters during the development of cancer. To achieve specific H2 S detection in cancer cells, we reported a biotin-guided NIR fluorescent sensor P1 targeting a cancer cell surface biomarker, based on the H2 S-specific thiolysis of the NBD–amine–hemicyanine conjugate. The probe showed a fast turn-on signal at 754 nm upon H2 S activation and good selectivity towards H2 S over millimolar levels of other biothiols. We successfully employed P1 to image endogenous H2 S and demonstrated its tumor-targeting ability in live cells. P1 could differentiate multiple cancer cells with various levels of H2 S from normal cells, indicating its potential for cancer imaging.