Enhanced β‐glucosidase in Western flower thrips affects its interaction with the redox‐based strategies of kidney beans under elevated CO2. (9th January 2023)

• Record Type: Journal Article
• Authors: Liu, Xiaowei; Wang, Yanhui; Liu, Hui; Huang, Xinyi; Qian, Lei; Yang, Baoqing; Xu, Yujing; Chen, Fajun
• Is Part Of: Plant, cell and environment. Volume 46:Number 3(2023)
• Page Start: 918
• Page End: 930
• Publication Date: 2023-01-09
• Subjects: climate change -- Frankliniella occidentalis -- Phaseolus vulgaris -- plant‐insect interaction -- salivary elicitor; Plant physiology -- Periodicals; Plant cells and tissues -- Periodicals; Plant communities -- Periodicals; 581.105
• DOI: 10.1111/pce.14534
• Languages: English
• ISSNs: 0140-7791

Abstract

Abstract: β‐Glucosidase is validated as an elicitor for early immune responses in plants and it was detected in the salivary glands of Frankliniella occidentalis in previous research. Seven differentially expressed genes encoding β‐glucosidase were obtained by comparing the transcriptomes of F. occidentalis adults grown under two different CO2 concentrations (800 vs. 400 ppm), which might be associated with the differences in the interaction between F. occidentalis adults and its host plant, Phaseolus vulgaris under different CO2 levels. To verify this speculation, changes in defense responses based on the production and elimination of reactive oxygen species (ROS) in P. vulgaris leaves treated with three levels of β‐glucosidase activity under ambient CO2 (aCO2 ) and elevated CO2 (eCO2 ) were measured in this study. According to the results, significantly higher levels of ROS were noticed under eCO2 compared to aCO2, which was caused by the increased β‐glucosidase activity in thrips due to increased cellulose content in P. vulgaris leaves under eCO2 . Together with the lower activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in injured leaves under eCO2, P. vulgaris leaves would be negatively affected on redox‐based defense by eCO2, thus facilitating thrips damage under climate change. Summary Statement: β‐Glucosidase secreted by the Frankliniella occidentalis adults could induce early immune responses in the leaves of their host plant, Phaseolus vulgaris based on reactive oxygen species (ROS) and antioxidant enzymes, which was significantly affected by CO2 level. The higher β‐glucosidase activity of F. occidentalis adults under elevated CO2 (eCO2 ), which was probably caused by the increased cellulose content in P. vulgaris leaves, would lead to increased ROS levels in P. vulgaris leaves infested with thrips under eCO2, and the activities of antioxidant enzymes for scavenging ROS damage would be correspondingly increased but their overall levels were still lower than that under ambient CO2, which was beneficial to the feeding damage of thrips