Covalent immobilization of lipase on an ionic liquid-functionalized magnetic Cu-based metal–organic framework with boosted catalytic performance in flavor ester synthesis. Issue 6 (18th January 2023)

• Record Type: Journal Article
• Authors: Suo, Hongbo; Geng, Huining; Zhang, Lu; liu, Guoyun; Yan, Hui; Cao, Rui; Zhu, Jiahao; Hu, Yi; Xu, Lili
• Is Part Of: Journal of materials chemistry. Volume 11:Issue 6(2023)
• Page Start: 1302
• Page End: 1311
• Publication Date: 2023-01-18
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/d2tb02246j
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : Lipase immobilized on an ionic liquid modified magnetic Cu-based metal–organic framework with enhanced catalytic performance in the synthesis of isoamyl acetate. Abstract : Enzymatic esterification plays an important role in the fields of chemistry and biotechnology. In this study, lipase was immobilized on an ionic liquid (IL)-modified magnetic metal–organic framework (MOF) and used to synthesize isoamyl acetate. The immobilized lipase (PPL-ILs/Fe3 O4 @MOF) showed 2.1-fold and 1.8-fold higher activity compared to the free and immobilized lipase without ILs (PPL-Fe3 O4 @MOF), respectively. In addition, the anti-denaturant ability and reusability of the PPL-ILs/Fe3 O4 @MOF were also higher than those of other samples. The ester yield reached 75.1% when the biocatalyst was used to synthesize isoamyl acetate in hexane. The synthesized supports supplied a good microenvironment for the immobilized lipase through multiple interactions. Results of the structural analysis showed that the conformation state of lipase molecules changed after immobilization. The magnetism of the prepared biocatalyst makes it easy to recycle so that PPL-ILs/Fe3 O4 @MOF maintained 70.2% of the initial activity after eight cycles. The prepared composite materials exhibited good potential in lipase immobilization with enhanced catalytic ability and stability.