An alternative σ factor σLsl regulates lincomycin production in Streptomyces lincolnensis. (1st December 2022)

• Record Type: Journal Article
• Authors: Tu, Bingbing; Mao, Yue; Wang, Ruida; Kang, Yajing; Ye, Jiang; Zhang, Huizhan; Wu, Haizhen
• Is Part Of: Journal of basic microbiology. Volume 63:issue 2(2023)
• Page Start: 190
• Page End: 199
• Publication Date: 2022-12-01
• Subjects: alternative σ factor -- lincomycin -- Streptomyces lincolnensis -- σLsl; Microbiology -- Periodicals; 579
• DOI: 10.1002/jobm.202200485
• Languages: English
• ISSNs: 0233-111X

Abstract

Abstract: Lincomycin produced by Streptomyces lincolnensis is a critical antibacterial antibiotic in the clinical. To further understand the regulatory mechanism of lincomycin biosynthesis, we identified an alternative σ factor, σ L sl, in Streptomyces lincolnensis NRRL 2936. Deletion of sigL sl resulted in an increase in cell growth but a decrease in lincomycin production. σ L sl boosted lincomycin biosynthesis by directly stimulating the transcription of four genes ( lmbD, lmbV, lmrC, and lmbU ) within the lincomycin biosynthetic lmb gene cluster. Besides, σ L sl participated in lincomycin biosynthesis by directly stimulating the transcription of mshC, a gene responsible for MSH synthesis. In conclusion, our findings demonstrated that σ L sl plays a direct regulatory role in lincomycin biosynthesis. This study extends the understanding of molecular mechanisms of lincomycin biosynthetic regulation.