Comparative analysis of dermal collagen and lipids in cereblon ablated mice using a multimodal nonlinear optical system. Issue 2 (20th September 2022) - Park, Chang Woo
Abstract: By utilizing a multimodal nonlinear optical system that combines coherent anti‐Stokes Raman scattering and second harmonic generation to investigate biological characteristics of dermal tissues ex vivo, we demonstrate the potential feasibility of using this optical approach as a powerful new investigative tool for future biomedical research. For this study, our optical system was utilized for the first time to analyze lipid and collagen profiles in cereblon knockout (KO) mouse skin, and we were able to discover significant alterations in the number of carbon–carbon double bonds (wild‐type vs. cereblon KO; NCC : 0.75 vs. 0.85) of skin fatty acids in triacylglycerides as well as changes in dermal collagen fibers (25% reduction in cereblon KO). By adopting our optical system to biological studies, we provide researchers with another diagnostic approach to validate their experimental results, which will significantly advance the state of biomedical research. Abstract : A multimodal nonlinear optical system was used for comparative analysis of lipids and collagen in cereblon‐depleted mouse skin tissues. We found more unsaturated fatty acids and decreased levels of dermal collagen fibers from cereblon knockout skin tissues. Our findings provide proof of principle that the optical tool can be an additional apparatus to investigate future biomedical research.