Wharton's jelly MSC-derived extracellular vehicles—loaded hyaluronic acid-alginate adhesives for treatment of osteoarthritis. (10th April 2023)

• Record Type: Journal Article
• Authors: Zhao, Yanhong; Zhao, Xige; Xu, Hainan; Xing, Yi; Wu, Tengling; Sun, Xun; Kuang, Mingjie; Ma, Xinlong; Liu, Wenguang; Yang, Qiang
• Is Part Of: Journal of materials science & technology. Volume 142(2023)
• Page Start: 240
• Page End: 252
• Publication Date: 2023-04-10
• Subjects: Extracellular vesicles -- Hydrogel -- Osteoarthritis -- Cartilage regeneration; Metals -- Periodicals; Materials science -- Periodicals; Materials science; Metals; Periodicals; 620.1105
• DOI: 10.1016/j.jmst.2022.09.061
• Languages: English
• ISSNs: 1005-0302

Abstract

Highlights: Synthesising an injectable and adhesive aldehyded sodium alginate crosslinked acylhydrazide-modified hyaluronic acid (ALG-CHO/HHA) hydrogel. Fabricating an injectable tissue adhesive hydrogel to load the therapeutic hWJMSC-sEVs to ensure their sustained release. The ALG-CHO/HHA-sEV platform meets the clinical demand for long-lasting repair with a single injection. Abstract: The repair of cartilage injury caused by osteoarthritis (OA) has long plagued clinicians. Mesenchymal stem cell (MSC)-derived small extracellular vesicles (sEVs) show great potential in cartilage regeneration and immunoregulation to realise cell-free therapy. However, unprotected sEVs are prone to be washed away by the flow of fluids and degraded in vivo . In this work, we successfully revealed that human Wharton's jelly of umbilical cord-derived MSC (hWJMSC) sEVs promote proliferation, inhibit apoptosis, and attenuate the inflammation of chondrocytes in vitro . In light of the long period required for cartilage regeneration, we synthesised an injectable and adhesive aldehyded sodium alginate crosslinked acylhydrazide-modified hyaluronic acid (ALG-CHO/HHA) hydrogel loaded with sEVs to boost the reparative effect of the sEVs. The hydrogel-bearing sEVs was injected into cartilage defects where they adhered to the cartilage surface. In this configuration, the sEVs were delivered in a sustainable manner with the degradation of the hydrogel at the injury sites, thus contributing to highly efficient cartilage repair by regulating the regenerative and immune microenvironment. The ALG-CHO/HHA-sEV platform meets the clinical demand for long-lasting repair with a single injection. Thus, this work provides a new idea and theoretical basis for the clinical application of sEVs in the treatment of OA.