Preparative separation of eight phenolic acids from Echinacea purpurea L. Moench using pH-zone-refining counter-current chromatography and evaluation of their immunomodulatory effects and synergistic potential. Issue 6 (23rd January 2023)

• Record Type: Journal Article
• Authors: Ma, Tianyu; Dong, Hongjing; Geng, Yanling; Guo, Lanping; Wang, Xiao
• Is Part Of: Analytical methods. Volume 15:Issue 6(2023)
• Page Start: 778
• Page End: 787
• Publication Date: 2023-01-23
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/d2ay01783k
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : Eight phenolic acids were isolated from Echinacea purpurea L. Moench using pH-ZRCCC. And the synergistic effect of caffeoyl tartaric acid, feruloyl tartaric acid, and cichoric acid on the inflammation was 94.91%. Abstract : Phenolic acids headed by cichoric acid in Echinacea purpurea L. Moench show remarkable immunomodulatory effects. In this study, pH-zone-refining counter-current chromatography with a two-phase solvent system composed of EtOAc–ACN–H2 O (4 : 1 : 5, v/v/v) (TFA (10 mM) in the upper phase and NH3 ·H2 O (30 mM) in the lower phase) was applied to the pre-segmentation enrichment of 3.5 g of a crude sample of Echinacea purpurea L. Moench. Then two fractions of highly concentrated samples were further separated using EtOAc–H2 O (1 : 1, v/v) and EtOAc– n -BuOH–ACN–H2 O (3 : 1 : 1 : 5, v/v/v/v) solvent systems (TFA (10 mM) in the upper phase and NH3 ·H2 O (10 mM) in the lower phase), separately. As a result, eight phenolic acids including cichoric acid were isolated successfully. Moreover, the immunomodulatory effects of the isolated compounds and the synergy of the binary and ternary compound combinations were first studied using CompuSyn software. And the ternary combination (caffeoyl tartaric acid + feruloyl tartaric acid + cichoric acid) showed a 94.91% synergistic inhibition rate of NO production released by LPS-induced RAW 264.7 cells. This study developed a strategy for the rapid preparative separation and purification of phenolic acids with complex components and large differences in content and provided a theoretical basis for the synergistic use of monomeric compounds in Echinacea purpurea L. Moench.