3D printed porous titanium filled with mineralized UV-responsive chitosan hydrogel promotes cell proliferation and osteogenesis in vitro. (10th April 2023)

• Record Type: Journal Article
• Authors: Yang, Jiazhao; Liu, Fan; Zhou, Changshun; Li, Hejie; Yang, Gaolin; Fang, Shiyuan; Lee, In-Seop; Liu, Yi; Bai, Hao; Chen, Cen
• Is Part Of: Journal of materials science & technology. Volume 142(2023)
• Page Start: 34
• Page End: 44
• Publication Date: 2023-04-10
• Subjects: Porous titanium -- Chitosan -- Ultraviolet (UV)-responsive -- Mineralization -- Osteogenesis; Metals -- Periodicals; Materials science -- Periodicals; Materials science; Metals; Periodicals; 620.1105
• DOI: 10.1016/j.jmst.2022.10.008
• Languages: English
• ISSNs: 1005-0302

Abstract

Highlights: UV-responsive chitosan (CSMA) could be in-situ mineralized by CO2 diffusion. Mineralized CSMA as filling material incorporated into internal pores of Ti implants. Ti implants filled with mineralized CSMA promoted osteogenesis of BMSCs. Abstract: The modification of 3D printed porous titanium (Ti), especially for the internal pore structure, is critical and has received more attention to promoting its osteogenesis for clinical use. Ultra-violet (UV) responsive chitosan (CSMA), as an injectable filling material, was firstly incorporated into porous Ti, and then CSMA was in-situ mineralized by carbon oxide (CO2 ) diffusion (CSMA/CaCO3 ). Their physical-chemical and biological properties were investigated in vitro . CaCO3 crystals within CSMA hydrogels were successfully deposited into pores of porous Ti, which exhibited favorable biocompatibility. Ti implants filled with CSMA/CaCO3 promoted adhesion and proliferation of bone mesenchymal stem cells (BMSCs). Moreover, Ti implant filled CSMA/CaCO3 hydrogels could increase alkaline phosphatase (ALP) activities, up-regulate osteopontin (OPN) and osteocalcin (OCN) expression levels, and enhance extracellular mineralization. 3D printed porous Ti filled with mineralized UV-responsive chitosan hydrogel could promote proliferation and osteogenesis of BMSCs, and have great potential for the modification of porous Ti implants in bone tissue engineering.