Circular RNA circPlce1 regulates innate immune response in miiuy croaker, Miichthys miiuy. Issue 133 (February 2023)

• Record Type: Journal Article
• Authors: Lv, Xing; Luo, Qiang; Xin, Shiying; Zheng, Weiwei; Xu, Tianjun; Sun, Yuena
• Is Part Of: Fish & shellfish immunology. Issue 133(2023)
• Publication Date: 2023-02
• Subjects: circRNA -- Immune response -- Innate immunity -- Fish; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2023.108561
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: In recent years, more and more researchers have devoted to the study of circular RNAs (circRNAs) in noncoding RNAs. As an important regulator in a variety of biological processes, circRNAs are relatively abundant in the study of mammals, while research in lower vertebrates is still lacking. In this study, we found a circRNA, circPlce1, related to innate immune response in Miichthys miiuy (miiuy croaker). The experimental results confirmed that circPlce1 could promote the production of antiviral genes and inflammatory response under the stimulation of poly (I: C) and LPS. We also confirmed that circPlce1 can promote NF-κB and IRF3 pathways through luciferase reporter assay experiment. In addition, we also found that circPlce1 can promote cell proliferation and improve cell viability. In conclusion, our results showed that circPlce1 plays an active role in regulating inflammatory response, cell proliferation and cell viability, providing a foundation for the study of the biological function of circRNAs in the innate immune response in teleost fish. Highlights: circPlce1 was up-regulated in M. miiuy spleen after stimulation with poly (I: C) and LPS. circPlce1 plays an active role in regulating antiviral and inflammatory response. circPlce1 regulates NF-κB and IRF3 signaling pathway.