Dynamic responses of the benthic bacterial community at the Western English Channel observatory site L4 are driven by deposition of fresh phytodetritus. (September 2015)

• Record Type: Journal Article
• Authors: Tait, Karen; Airs, Ruth L.; Widdicombe, Claire E.; Tarran, Glen A.; Jones, Mark R.; Widdicombe, Stephen
• Is Part Of: Progress in oceanography. Volume 137:Part B(2015:Sep.)
• Page Start: 546
• Page End: 558
• Publication Date: 2015-09
• Subjects: Oceanography -- Periodicals; 551.4605
• DOI: 10.1016/j.pocean.2015.04.020
• Languages: English
• ISSNs: 0079-6611

Abstract

Highlights: The response of a benthic bacterial community to a phytoplankton bloom was studied. Phytoplankton material was heavily degraded only when it reached the sediment. The abundance of benthic bacteria and chlorophyll were correlated. Bacterial community composition shifted rapidly through-out the bloom. This was due to an increase in relative abundance of members of the Flavobacteria . Abstract: The impact of the seasonal deposition of phytoplankton and phytodetritus on surface sediment bacterial abundance and community composition was investigated at the Western English Channel site L4. Sediment and water samples were collected from January to September in 2012, increasing in frequency during periods of high water column phytoplankton abundance. Compared to the past two decades, the spring bloom in 2012 was both unusually long in duration and contained higher than average biomass. Within spring months, the phytoplankton bloom was well mixed through the water column and showed accumulations near the sea bed, as evidenced by flow cytometry measurements of nanoeukaryotes, water column chlorophyll a and the appearance of pelagic phytoplankton at the sediment. Measurements of chlorophyll and chlorophyll degradation products indicated phytoplankton material was heavily degraded after it reached the sediment surface: the nature of the chlorophyll degradation products (predominantly pheophorbide, pyropheophorbide and hydroxychlorophyllone) was indicative of grazing activity. The abundance of bacterial 16S rRNA genes g −1 sediment (used as a proxy for bacterial biomass) increased markedly with the onset of the phytoplankton bloom, and correlated with measurements of chlorophyll at the surface sediment. Together, this suggests that bacteria may have responded to nutrients released via grazing activity. In depth sequencing of the 16S rRNA genes indicated that the composition of the bacterial community shifted rapidly through-out the prolonged spring bloom period. This was primarily due to an increase in the relative sequence abundance of Flavobacteria .