MOF-derived hierarchically ordered porous carbon for the immobilization of Eversa® Transform 2.0 and its post-immobilization hydrophobization in biodiesel production. (1st May 2023)

• Record Type: Journal Article
• Authors: Zhou, Hao; Dai, Lingmei; Liu, Dehua; Du, Wei
• Is Part Of: Fuel. Volume 339(2023)
• Publication Date: 2023-05-01
• Subjects: Immobilization -- Hierarchically ordered porous structure -- MOF-derived carbon -- Metal organic frameworks (MOFs) -- Stability -- Zeolitic imidazolate frameworks (ZIF-8); Fuel -- Periodicals; Coal -- Periodicals; Coal; Fuel; Periodicals; 662.6
• DOI: 10.1016/j.fuel.2023.127426
• Languages: English
• ISSNs: 0016-2361

Abstract

Highlights: We reported a prospective strategy of immobilizing lipase onto MOF-derived hierarchically ordered porous carbon. 3DOM-C, derived from 3D ordered macroporous ZIF-8, performed superiorly in lipase immobilization and biodiesel production. A facile post-immobilization hydrophobization strategy was proposed and a much higher reaction rate was achieved. Abstract: MOF-derived carbon has extraordinary advantages including highly ordered morphology inherited from MOFs and improved chemical stability arising from its carbon nature, which may be a promising immobilized enzyme carrier. Herein, we prepared two MOF-derived carbon materials of different structures for the immobilization of lipase from Eversa® Transform 2.0 and systematically evaluated their application potential in biodiesel production system. Compared with C-C (derived from conventional microporous ZIF-8), 3DOM-C (derived from 3D ordered macro-microporous ZIF-8) exhibited superior immobilization performance with an 145.1% increase in enzyme loading and an 130.2% increase in activity. Moreover, lipase immobilized on 3DOM-C (3DOM-C@TLL) showed better catalytic performance than C-C@TLL, with faster conversion rate in lipase-mediated biodiesel production. Remarkably, the higher surface hydrophobicity of the 3DOM-C@TLL was proved to be the key factor in its improved catalytic performance. Meanwhile, we found that the affinity between immobilized enzyme molecules and water significantly weakened the hydrophobicity of C-C@TLL and 3DOM-C@TLL, which could be of adverse effect on their application in biodiesel production. To enhance the hydrophobicity of immobilized lipase biocatalyst, a facile post-immobilization hydrophobic modification strategy was proposed by coating the 3DOM-C@TLL with polydimethylsiloxane (PDMS). The as obtained super hydrophobic 3DOM-C@TLL@PDMS biocatalyst showed excellent catalytic performance in biodiesel production, with a much higher initial reaction rate, which was 3.3 folds of that catalyzed by C-C@TLL, and 1.8 folds of that catalyzed by 3DOM-C@TLL. This work shed light on the great potential of constructing MOF-derived hierarchically ordered porous carbon for enzyme immobilization in extensive practical applications.