Lipids and membrane-associated proteins in autophagy. Issue 7 (5th November 2020)

• Record Type: Journal Article
• Authors: Li, Linsen; Tong, Mindan; Fu, Yuhui; Chen, Fang; Zhang, Shen; Chen, Hanmo; Ma, Xi; Li, Defa; Liu, Xiaoxia; Zhong, Qing
• Is Part Of: Protein & cell. Volume 12:Issue 7(2021)
• Page Start: 520
• Page End: 544
• Publication Date: 2020-11-05
• Subjects: autophagy -- membrane-associated proteins -- membrane-associated biochemistry assays -- ATG2 -- ESCRT -- lipid transfer -- elongation -- scission -- fusion; Proteins -- Periodicals; Cells -- Periodicals; Cytology -- Periodicals; 572.6
• DOI: 10.1007/s13238-020-00793-9
• Languages: English
• ISSNs: 1674-800X

Abstract

Abstract: Autophagy is essential for the maintenance of cellular homeostasis and its dysfunction has been linked to various diseases. Autophagy is a membrane driven process and tightly regulated by membrane-associated proteins. Here, we summarized membrane lipid composition, and membrane-associated proteins relevant to autophagy from a spatiotemporal perspective. In particular, we focused on three important membrane remodeling processes in autophagy, lipid transfer for phagophore elongation, membrane scission for phagophore closure, and autophagosome-lysosome membrane fusion. We discussed the significance of the discoveries in this field and possible avenues to follow for future studies. Finally, we summarized the membrane-associated biochemical techniques and assays used to study membrane properties, with a discussion of their applications in autophagy.