Increased levels of microRNA‐320 in blood serum and plasma is associated with imminent and advanced lung cancer. Issue 2 (27th November 2022)

• Record Type: Journal Article
• Authors: Nøst, Therese Haugdahl; Skogholt, Anne Heidi; Urbarova, Ilona; Mjelle, Robin; Paulsen, Erna‐Elise; Dønnem, Tom; Andersen, Sigve; Markaki, Maria; Røe, Oluf Dimitri; Johansson, Mikael; Johansson, Mattias; Grønberg, Bjørn Henning; Sandanger, Torkjel Manning; Sætrom, Pål
• Is Part Of: Molecular oncology. Volume 17:Issue 2(2023)
• Page Start: 312
• Page End: 327
• Publication Date: 2022-11-27
• Subjects: diagnostic markers -- lung cancer -- MiRNA -- peripheral blood -- pre‐diagnostic markers; Cancer -- Molecular aspects -- Periodicals; 616.994005
• DOI: 10.1002/1878-0261.13336
• Languages: English
• ISSNs: 1574-7891

Abstract

Abstract : Lung cancer (LC) incidence is increasing globally and altered levels of microRNAs (miRNAs) in blood may contribute to identification of individuals with LC. We identified miRNAs differentially expressed in peripheral blood at LC diagnosis and evaluated, in pre‐diagnostic blood specimens, how long before diagnosis expression changes in such candidate miRNAs could be detected. We identified upregulated candidate miRNAs in plasma specimens from a hospital‐based study sample of 128 patients with confirmed LC and 62 individuals with suspected but confirmed negative LC (FalsePos). We then evaluated the expression of candidate miRNAs in pre‐diagnostic plasma or serum specimens of 360 future LC cases and 375 matched controls. There were 1663 miRNAs detected in diagnostic specimens, nine of which met our criteria for candidate miRNAs. Higher expression of three candidates, miR‐320b, 320c, and 320d, was associated with poor survival, independent of LC stage and subtype. Moreover, miR‐320c and miR‐320d expression was higher in pre‐diagnostic specimens collected within 2 years of LC diagnosis. Our results indicated that elevated levels of miR‐320c and miR‐320d may be early indications of imminent and advanced LC. Abstract : We applied a novel approach of identifying early markers of lung cancer using both diagnostic and pre‐diagnostic blood specimens. We detected elevated levels of miR‐320c and miR‐320d in blood up to 2 years prior to the lung cancer diagnosis. These two miRNAs were identified as the most promising markers and may be early indications of imminent and advanced lung cancer.