Co‐exposure to molybdenum and cadmium triggers pyroptosis and autophagy by PI3K/AKT axis in duck spleens. Issue 3 (18th November 2022)

• Record Type: Journal Article
• Authors: Chu, Xuesheng; Dai, Xueyan; Pu, Wenjing; Guo, Huiling; Huang, Gang; Huang, Bingyan; Cui, Ting; Zhang, Caiying
• Is Part Of: Environmental toxicology. Volume 38:Issue 3(2023)
• Page Start: 635
• Page End: 644
• Publication Date: 2022-11-18
• Subjects: autophagy -- cadmium -- molybdenum -- PI3K/AKT axis -- pyroptosis -- spleen; Water quality bioassay -- Periodicals; Water -- Pollution -- Toxicology -- Periodicals; Microbiological assay -- Periodicals; Toxicity testing -- Periodicals; Environmental toxicology -- Periodicals; Environmental Pollution -- Periodicals; Environmental Pollutants -- Periodicals; Environmental Monitoring -- Periodicals; Écotoxicologie -- Périodiques; Pollution -- Périodiques; 615.902
• DOI: 10.1002/tox.23712
• Languages: English
• ISSNs: 1520-4081

Abstract

Abstract: Excessive amounts of molybdenum (Mo) and cadmium (Cd) are toxicant, but their combined immunotoxicity are not clearly understood. To estimate united impacts of Mo and Cd on pyroptosis and autophagy by PI3K/AKT axis in duck spleens, Mo or/and Cd subchronic toxicity models of ducks were established by feeding diets with different dosages of Mo or/and Cd. Data show that Mo or/and Cd cause oxidative stress by increasing MDA concentration, and decreasing T‐AOC, CAT, GSH‐Px and T‐SOD activities, restrain PI3K/AKT axis by decreasing PI3K, AKT, p‐AKT expression levels, which evokes pyroptosis and autophagy by elevating IL‐1β, IL‐18 concentrations and NLRP3, Caspase‐1, ASC, GSDME, GSDMA, NEK7, IL‐1β, IL‐18 expression levels, promoting autophagosomes, LC3 puncta, Atg5, LC3A, LC3B, LC3II/LC3I and Beclin‐1 expression levels, and reducing expression levels of P62 and Dynein. Furthermore, the variations of abovementioned indexes are most pronounced in co‐treated group. Overall, results reveal that Mo or/and Cd may evoke pyroptosis and autophagy by PI3K/AKT axis in duck spleens. The association of Mo and Cd exacerbates the changes.