De novo bio‐production of odd‐chain fatty acids in Saccharomyces cerevisiae through a synthetic pathway via 3‐hydroxypropionic acid. Issue 3 (10th December 2022)

• Record Type: Journal Article
• Authors: Qi, Nailing; Ding, Wentao; Dong, Genlai; Wang, Zhihui; Shi, Shuobo
• Is Part Of: Biotechnology and bioengineering. Volume 120:Issue 3(2023)
• Page Start: 852
• Page End: 858
• Publication Date: 2022-12-10
• Subjects: 3‐hydroxypropionic acid -- metabolic engineering -- odd‐chain fatty acids -- Saccharomyces cerevisiae -- β‐alanine pathway; Biotechnology -- Periodicals; Bioengineering -- Periodicals; 660.6
• DOI: 10.1002/bit.28297
• Languages: English
• ISSNs: 0006-3592

Abstract

Abstract: Odd‐chain fatty acids (OCFAs) and their derivatives have attracted increasing attention due to their wide applications in the chemical, fuel, and pharmaceutical industry. However, most natural fatty acids are even‐chained, and OCFAs are rare. In this work, a novel pathway was designed and established for de novo synthesis of OCFAs via 3‐hydroxypropionic acid (3‐HP) as the intermediate in Saccharomyces cerevisiae . First, the OCFAs biosynthesis pathway from 3‐HP was confirmed, followed by an optimization of the precursor 3‐HP. After combining these strategies, a de novo production of OCFAs at 74.8 mg/L was achieved, and the percentage of OCFAs in total lipids reached 20.3%, reaching the highest ratio of de novo‐produced OCFAs. Of the OCFAs produced by the engineered strain, heptadecenoic acid (C17:1) and heptadecanoic acid (C17:0) accounted for 12.1% and 7.6% in total lipid content, respectively. This work provides a new and promising pathway for the de novo bio‐production of OCFAs.