Common carp Peptidoglycan Recognition Protein 2 (CcPGRP2) plays a role in innate immunity for defense against bacterial infections. Issue 133 (February 2023)

• Record Type: Journal Article
• Authors: Bai, Linyi; Zhou, Yuan; Sheng, Chen; Yin, Yizhi; Chen, Yanru; Ding, Xinli; Yu, Guanliu; Yang, Guiwen; Chen, Lei
• Is Part Of: Fish & shellfish immunology. Issue 133(2023)
• Publication Date: 2023-02
• Subjects: Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2023.108564
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: PGRP is a family of pattern recognition molecules of the innate immune system. PGRPs are conserved from insects to mammals and have diverse functions in antimicrobial defense. Here we cloned a common carp PGRP ortholog, CcPGRP2 containing a conserved C-terminal PGRP domain. We tested the expression levels of CcPGRP2 in the liver, spleen, kidney, foregut, midgut, and hindgut of the highest level in the liver. The expression of CcPGRP2 upregulated in common carp infected with Aeromonas hydrophila ( A. hydrophila ) or Staphylococcus aureus ( S. aureus ). Recombinant CcPGRP2 protein expressed in Escherichia coli ( E. coli ) system and the purified CcPGRP2 could maintain the integrity of intestinal mucosa of common carp infected with A. hydrophila . In addition, CcPGRP2 could agglutinate or bind both gram-positive and gram-negative bacteria in a Zn 2+ -dependent manner. CcPGRP2 has a stronger agglutination and bacterial binding ability in gram-positive bacteria than in gram-negative bacteria. It is perhaps because CcPGRP2 could bind peptidoglycan (PGN) with a higher degree to lipopolysaccharide (LPS). And CcPGRP2 shows antimicrobial activities in the presence of Zn 2+ . Our results of CcPGRP2 provided new insight into the function of PGRP in the innate immunity of the common carp. Highlights: CcPGRP2 was expressed in the liver, spleen, kidney, foregut, midgut, and hindgut of common carp. CcPGRP2 could bind LPS and PNG, agglutinate bacteria and inhibit bacterial growth in a Zn 2+ -dependent manner. CcPGRP2 could maintain the integrity of the intestinal mucosa infected by A. hydrophila. CcPGRP2 plays an important role in the natural immunity of common carp.