IL-27 suppresses spring viremia of carp virus replication in zebrafish. Issue 133 (February 2023)

• Record Type: Journal Article
• Authors: Shi, Yanjie; Chen, Kangyong; Zhao, Xin; Lu, Yanan; Huang, Wenji; Guo, Jiahong; Ji, Ning; Jia, Zhao; Xiao, Hehe; Dang, Huifeng; Zou, Jun; Wang, Junya
• Is Part Of: Fish & shellfish immunology. Issue 133(2023)
• Publication Date: 2023-02
• Subjects: Interleukin-27A -- EBI3 -- Antiviral response -- SVCV -- Zebrafish; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2023.108530
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Interleukin (IL) 27 is a member of the IL-12 family and is a heterodimeric cytokine composed of IL-27A and Epstein-Barr virus-induced 3 (EBI3). It plays an important role in regulating inflammation and cancer progression. IL-27A not only functions by dimerizing with EBI3 but also acts alone. Here, we report that IL-27A and EBI3 suppress spring viremia of carp virus (SVCV) replication in zebrafish. Expression analysis reveals that il-27a and ebi3 were significantly upregulated in the ZF4 cells by SVCV and poly(I:C), and in the zebrafish caudal fin (ZFIN) cells overexpressed with SVCV genes. Interestingly, il-27a and ebi3 were not modulated by IFNφ1, indicating that they are not IFN stimulated genes (ISGs). Furthermore, overexpression of IL-27A and EBI3 alone inhibited SVCV replication in the EPC cells, but less potent than co-expression of IL-27A and EBI3. Intriguingly, IL-27A could not induce the expression of irf3, ifn, isg15 and mx1 . Taken together, our results demonstrate that IL-27A and EBI3 activate innate antiviral response in an IFN independent manner in zebrafish. Highlights: Zebrafish il-27a and ebi3 were induced by virus but not type I IFN. IL-27A did not upregulate irf3, ifn, isg15 and mx1. IL-27A and EBI3 inhibited replication of spring viremia of carp virus.