Supersensitive detection of single-histamine molecule on nanoplates by turn-on small molecule fluorescence sandwich immunoassay. Issue 4 (24th January 2023)
- Record Type:
- Journal Article
- Title:
- Supersensitive detection of single-histamine molecule on nanoplates by turn-on small molecule fluorescence sandwich immunoassay. Issue 4 (24th January 2023)
- Main Title:
- Supersensitive detection of single-histamine molecule on nanoplates by turn-on small molecule fluorescence sandwich immunoassay
- Authors:
- Lee, Seungah
Lee, Junghwa
Kang, Seong Ho - Abstract:
- Abstract : Turn-on small molecule fluorescence sandwich immunoassay. Abstract : We develop a supersensitive "turn-on format" fluorescence sandwich immunoassay for detecting small single molecules. Gold nanoplate-based biotin antibodies and streptavidin–fluorophores were used instead of streptavidin–horseradish peroxidase reacting with a biotin tracer in a microplate-based competitive enzyme-linked immunosorbent assay (ELISA). Our platform showed a low detection limit of 5 zeptomolar (5 × 10 –21 M), 5.4 × 10 10 times higher detection sensitivity than the conventional tune-off format ELISA.
- Is Part Of:
- Analyst. Volume 148:Issue 4(2023)
- Journal:
- Analyst
- Issue:
- Volume 148:Issue 4(2023)
- Issue Display:
- Volume 148, Issue 4 (2023)
- Year:
- 2023
- Volume:
- 148
- Issue:
- 4
- Issue Sort Value:
- 2023-0148-0004-0000
- Page Start:
- 714
- Page End:
- 718
- Publication Date:
- 2023-01-24
- Subjects:
- Chemistry, Analytic -- Periodicals
543 - Journal URLs:
- http://pubs.rsc.org/en/journals/journalissues/an?e=1#!issueid=an139020&type=current&issnprint=0003-2654 ↗
http://www.rsc.org/ ↗ - DOI:
- 10.1039/d2an01734b ↗
- Languages:
- English
- ISSNs:
- 0003-2654
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 0893.000000
British Library DSC - BLDSS-3PM
British Library STI - ELD Digital store - Ingest File:
- 25703.xml