Phenolic constituents, antioxidant and α-amylase inhibitory activities of Pulicaria vulgaris growing in Tunisia: an in vitro and in silico study. (2nd January 2023)

• Record Type: Journal Article
• Authors: Jlizi, Salma; Lazrag, Houda; Oulad El Majdoub, Yassine; Zardi-Bergaoui, Afifa; Cacciola, Francesco; Mondello, Luigi; Harrath, Abdel Halim; Ben Jannet, Hichem
• Is Part Of: Plant biosystems. Volume 157:Number 1(2023)
• Page Start: 61
• Page End: 70
• Publication Date: 2023-01-02
• Subjects: Pulicaria vulgaris -- aerial parts -- polyphenolic compounds -- HPLC-PDA/ESI-MS -- antioxidant -- anti-α-amylase -- molecular docking; Botany -- Periodicals; Biological systems -- Periodicals; Plants -- Periodicals; 581
• DOI: 10.1080/11263504.2022.2089760
• Languages: English
• ISSNs: 1126-3504

Abstract

Abstract: The present research aimed to establish the polyphenolic profile and to appraise the antioxidant and anti-α-amylase activities of the EtOAc and BuOH extracts from the aerial parts of Pulicaria vulgaris. Total phenolics and flavonoids of both extracts were quantified. A total of 4 and 19 polyphenolic compounds were identified in the EtOAc (major component: methylcatechin: 261.51 ± 0.03 g kg −1 ) and BuOH extracts (major component: 1, 3- O -caffeoylquinic acid:12.95 ± 0.12 g kg −1 ), respectively, by HPLC-PDA/ESI-MS analysis. P. vulgaris extracts showed interesting in vitro antioxidant properties. The EtOAc and BuOH extracts exhibited an α-amylase inhibitory action (IC50 values of 10.0 and 7.5 μgmL −1, respectively). It has been shown that the EtOAc extract produced an uncompetitive inhibition against α-amylase, while the BuOH extract inhibited this enzyme in mixed manner. The molecular docking analysis of the major constituents of BuOH extract showed a low binding energy, a correct mode of interaction in the active pocket of the enzyme, and an ability to interact with the key residues of glycosidic cleavage (GLU-230 and ASP-206), which can explain the strong inhibitory effect of α-amylase established by the BuOH extract. This is the first report on the polyphenolic composition, antioxidant and anti-α-amylase activities of the extracts from the aerial parts of P. vulgaris .