Transcriptome analysis of Macrobrachium rosenbergii hemocytes reveals in-depth insights into the immune response to Vibrio parahaemolyticus infection. Issue 133 (February 2023)

• Record Type: Journal Article
• Authors: Zhan, Fanbin; Li, Yanan; Shi, Fei; Lu, Zhijie; Yang, Minxuan; Li, Qingqing; Lin, Li; Qin, Zhendong
• Is Part Of: Fish & shellfish immunology. Issue 133(2023)
• Publication Date: 2023-02
• Subjects: Macrobrachium rosenbergii -- Vibrio parahaemolyticus -- Hemocytes -- Immune genes; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2023.108533
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Macrobrachium rosenbergii as one of the common freshwater prawn species in Southeast Asia, which breeding industry is seriously threatened by vibriosis and causes high mortality. In this study, the RNA-seq was employed for assessing the M. rosenbergii hemocytes transcriptomes following Vibrio parahaemolyticus challenge. After challenge for 6 h (h), there were overall 1849 DEGs or differentially expressed genes, including 1542 up-regulated and 307 down-regulated genes, and there was a total of 1048 DEGs, including 510 up-regulated genes and 538 down-regulated genes, after challenge for 12 h. Mitogen-activated protein kinase (MAPK) immune-related pathways, Toll, immune deficiency (IMD), and Janus kinase (JAK)/signal transducer and activator of transcription (STAT) were among the immune pathways where a lot of the DEGs were connected. The expression patterns of 18 chosen immune-related genes were examined utilizing qRT-PCR or quantitative real-time polymerase chain reaction, which revealed that the V. parahaemolyticus infection activated the M. rosenbergii's immune response. Permutational multivariate analysis of variance (PERMANOVA) showed that V. parahaemolyticus infection modulated immune regulation and apoptosis pathways. The gathered information provided new insight into M. rosenbergii's immunity and suggested a novel approach to fight against bacterial infection. Highlights: Transcriptomic database of Macrobrachium rosenbergii challenged with Vibrio parahaemolyticus. The infection of V. parahaemolyticus modulated immune regulation and apoptosis pathways. Provide a new light on the immunity of M. rosenbergii for fighting against bacterial infection.