A roadmap for interpreting 13C metabolite labeling patterns from cells. (August 2015)

• Record Type: Journal Article
• Authors: Buescher, Joerg M; Antoniewicz, Maciek R; Boros, Laszlo G; Burgess, Shawn C; Brunengraber, Henri; Clish, Clary B; DeBerardinis, Ralph J; Feron, Olivier; Frezza, Christian; Ghesquiere, Bart; Gottlieb, Eyal; Hiller, Karsten; Jones, Russell G; Kamphorst, Jurre J; Kibbey, Richard G; Kimmelman, Alec C; Locasale, Jason W; Lunt, Sophia Y; Maddocks, Oliver DK; Malloy, Craig; Metallo, Christian M; Meuillet, Emmanuelle J; Munger, Joshua; Nöh, Katharina; Rabinowitz, Joshua D; Ralser, Markus; Sauer, Uwe; Stephanopoulos, Gregory; St-Pierre, Julie; Tennant, Daniel A; Wittmann, Christoph; Vander Heiden, Matthew G; Vazquez, Alexei; Vousden, Karen; Young, Jamey D; Zamboni, Nicola; Fendt, Sarah-Maria;
• Is Part Of: Current opinion in biotechnology. Volume 34(2015)
• Page Start: 189
• Page End: 201
• Publication Date: 2015-08
• Subjects: Biotechnology -- Periodicals; 660.6
• DOI: 10.1016/j.copbio.2015.02.003
• Languages: English
• ISSNs: 0958-1669

Abstract

Graphical abstract: Highlights: 13 C tracer analysis can be used to probe intracellular metabolism in cells. Steady state labeling patterns can quantify nutrient contributions. Steady state labeling patterns can inform about pathway activities. Dynamic labeling patterns combined with metabolite levels can quantify some fluxes. Analysis of multiple metabolites and various tracers enhances information output. Abstract : Measuring intracellular metabolism has increasingly led to important insights in biomedical research. 13 C tracer analysis, although less information-rich than quantitative 13 C flux analysis that requires computational data integration, has been established as a time-efficient method to unravel relative pathway activities, qualitative changes in pathway contributions, and nutrient contributions. Here, we review selected key issues in interpreting 13 C metabolite labeling patterns, with the goal of drawing accurate conclusions from steady state and dynamic stable isotopic tracer experiments.